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- QUESTION 17 OL NEere interested in generating a PCR amplicon including the bracketed sequence below. Which of the following sequences would be canen hybridizing (annealing) with the target AND would also serve to generate a copy of the bracketed region of interest? 5'-AATCGT[AGCAGCAGCAGTGGCT]A AGCT-3 3' -TTAGCA[TC GTC GTC GTC ACC G A] TTCG A - 5' 3-TTAGC-S S-AATCG-3 OSAAGCT-3 5-AGCTT-3 5-GCTAA-3 5-TCGAA-3 QUESTION 18 Vhich of the following is/are true regarding the enzvme PRIMASE? Save and Submit to save and submit. Click Save All Answers to save all answers.Question 4 "In this mutation, the AGC is transcribed into UAG. " O missense mutation O nonsense mutation O silent mutation O frameshift mutationQuestion 3 Bacteria defend against phages by Natural selection favors mutation can not be recognized by virus. O all of these except DNA replication O restriction enzymes CRISPR-Cas system O DNA replication
- QUESTION 5 Based on the plasmid shown, what will you have to use to to distinguish and isolate the bacteria that successfully took up the plasmid? O Lactose O Tetracycline O Ampicillin O UV light to identify the glowing colonies O XgalQuestion 19 A transversion mutation would be replacing T by: A either A or G BU (c) T D) cQUESTION 6 To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for your reaction. Which of the following primer pairs would probably work for verifying your insert is actually present in the plasmid? 5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGG TTTACTATCCTGATGAAATTTT (Very long, but a bunch of nucleotides her e).... TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTGATGAGGATGACTC TGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3' Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 O A. Reverse: 5' CAA ATT ACA TTA CAC ATA A 3' Tm= 47.4 Forward: 5' ATG TTT ATT TTC TTA TTA TTT 3' Tm= 47.1 C O B. Reverse: 5' TAT GTG TAA TGT AAT TTG ACA CCC 3' Tm3 58.4 Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm3 59.8 OC. Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4 Forward: 5' GGT CAC TAC CAC TAG TGA GAG 3' 59.4 C O D. Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG…
- QUESTION 14 The Hershey and Chase experiments, in which radioactive phosphorus (32P) and radioactive sulfur (35S) were used, demonstrated that Oa. DNA labeled with 32P is transferred from the bacteriophage to the virus O b.DNA may be the hereditary material, although bacteriophages transfer both DNA and proteins into their hosts Oc. proteins labeled with 35S become deactivated and unable to be transferred O d. DNA labeled with 35S and proteins labeled with 32P can be traced over the course of an experiment O e. bacteriophages transfer their DNA, not their coat proteins, into their hosts QUESTION 15 Which of the following makes its own food using energy and simple raw materials it obtains from nonbiological sources? O a. Cell O b. Producer O C. Protist O d. Consumer O e. Nutrient QUESTION 16 A salt will dissolve in water to form O a bases O b.acids O c. buffers O d. jons other than H* and OH O e.only hydrogen and oxygen bonds QUESTION 17 The solvent, cohesive, and temperature…QUESTION 26 3.0 The creation of knockout (or knockin) mice involves all but one of the following O A. recombination between the vector and the chromosome O B. a drug selection marker carried on the vector O C. Introduction of the knockout/knockin gene by microinjection into a fertilized oocyte O D. growth of stem cells in culture O E. mating of mice in the first generation to one another to obtain a purebred population. QUESTION 27 3.0 A restriction fragment length polymorphism (RFLP) can result from: O A. A base change that results in the loss of a restriction site O B. Deletion or insertion of DNA within a restriction fragment O C.A change in hybridization stringency O D.A base change that creates a new restriction site O E. C. only O F. A., B., and D.Question 28 Imagine that vou are working in a lab studying the SARS-COV-2 virus and you are trying to do an Avery-McLeod-McCarty type experiment to determine the genetic material of this virus. You know that if you transduce a mixture of macromolecules from viral isolates (taken from buman nas swabsinto human cultured cells, new infectious viral particles are made. You perform three experiments: 1. You treat the mixture with protease, then observe synthesis of viral particles. 2. You treat the mixture with RNase and observe no synthesis of viral particles 3 You treat the mixture with DNase and observe synthesis of viral particles. How do you interpret these results? The SARS-CoV-2 virus uses RNA as its genetic material. There was likely an error in the experiment because DNase treatment should have had the opposite result. II. II. The SARS-CoV-2 virus uses both proteins and DNA as its genetic material. Iy Avery, McLeod and McCarty tried this with SARS-CoV-2 and obtained different…
- Question 28 Binding of transcription activator protein O Gene is switched ON O Gene is switched OFF O Does NOT affect the gene expressionQuèstion 34 Enzyme that joins the DNA fragments O Recombinant DNA Technology O Restriction enzymes O Ligase O Palindromic sequencesQuestion 4 1 pts Original DNA strand: ATT GAG CC Mutated DNA strand: ATT GAG CT What type of mutation does the example above demonstrate? O Substitution O The example above does not demonstrate any mutation at all. Deletion O There is not enough information to determine which type of mutation is demonstrated. O Insertion