Question 16 In a reverse phase chromatography setup, which of the following compounds will have the HIGHEST retention time? O water O isopropanol O toluene O ethanol
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- QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesQno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatography
- Based on the given chromatograph, answer the following questions:1) Which of the diet sodas have the highest concentration of caffeine? Which standard best reflects this concentration?2) Which of the diet sodas have the lowest concentration of aspartame? Which standard best reflects this concentration?3) What is the approximate retention time of benzoate?▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPAAnswer sections C, D and E in the same answer book. Section D (Chromatography) Question 8 The following data were obtained for the analysis of a mixture of compounds (A-D) by HPLC: Peak Retention time (min) | Width of peak base (min) non-retained 2.1 A 6.4 10.51 B 11.3 1.03 C 13.1 1.25 D 21.6 1.62 a) Using peak C. calculate the number of plates (N) in this column. b) For peak D, calculate the capacity factor (k'). c) For peak B and C, calculate the resolution (R).
- would column chromatography separate ketone and alcoholIn a reverse phase chromatography setup, which of the following compounds will have the HIGHEST retention time? water toluene isopropanol ethanolanch of the following components cannot be detected by gas chromatography? 4 Essential oils Alcohols b) Volatile compounds d) Non-volatile compounds 13- is measured as time elapsed from the point of injection to the peak maximum? a)-Column efficiency d)- Delectability c)-Retention time 14- Which of the following can be used to detect the components in gas chromatography a) non-volatile compounds b) volatile compounds c) Salts d) b&c 15-Which of the following methods is often referred to as mechanical separation processes. a)-Gas Chromatography b)-column chromatography c)- Sedimentation 16- For obtaining better resolution in TLC. and then developed with a second solvent at right angles to the first a) Stepwise b) Descending e) Ascending 17- This is defined as transport of electrically charged particles in a direct-current electric field a) ion exchange chromatography b) Electrophoresis c) ultracentrifuge d) exclusion 18-This figure indicates to schematic Diagram of a) Gas chromatography…
- Question 7 The relative peak areas and retention for a mixture of five fatty acids separated by GC is given in the table shown below. Based on this information, what percentage of the total content is comprised of FA 3? Retention time Peak Area Fatty Acid 1 4.176 123314 2 6.004 35669 3 7.542 78968 4 9.256 16122 5 13.196 28074 OA. 43.7% O B. 30.0% O C. 47.3% O D. 33.7%Why chromatography is an important tool for analysis of organic compounds?▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? ▪ A) methanol B) Ethanol ▪ C) IPA