Protein A binds to molecule Z with a Ka of 15.0 x 109. If the concentration of molecule Z is 30.0 nmol L1, calculate the fraction of Protein A bound to molecule Z. (Answer as a decimal fraction, not as a %.)
Q: The predominate form of caffeine at pH 9.4 is neutrally charged. If nitromethane, a compound often…
A: Caffeine is a member of alkaloid family. It is a group of compounds obtained from plants and whose…
Q: Look up the pKa values for trimethylamine, dimethylamine, methylamine, and ammonia. Predict the…
A: pKb tells about the basicity of any organic base. Higher the pKb value, lower would be the basicity.
Q: 6. If there is one monolayer coverage of organic contamination on the wafer, how much is that…
A: In order to solve this question, we will be substituting one silicon atom with a carbon atom on the…
Q: Predict the order of elution of benzyl alcohol (C6H5CH2OH), benzaldehyde(C6H5CHO), benzoic acid…
A: Elution is dependent on the polarity of the compound. They are inversely related to each other. If…
Q: Describe the mechanism of end point detection by EBT.
A: Answer - EBT. - Eriochrome Black T is a complexometric indicator that is used in complexometric…
Q: The pKa values of the conjugate acids of 2-aminopyridine (6.71) and 4- aminopyridine (9.11) are as…
A: It is given that 2-amino pyridine conjugate acid has a pKa value of 6.71 and 4-aminopyridine…
Q: Acetone is the compound used to measure tM- : Write down the order of polarity of the compounds from…
A: Polarity is determined by Number of Polar group present in the molecule.
Q: The relative electrophoretic mobilities of a 30-kDa protein and a 92-kDa protein used as standards…
A:
Q: Draw out the structure of the main form of p-nitrophenol at pH 5. Also, draw an example of a plot…
A:
Q: A sample of water from the North Sea was analysed by ion-chromatography and the sulfate…
A:
Q: Which of the following best describes the goal of an extraction procedure? a. the isolation of a…
A: We use various techniques to separate a compound or molecules from the mixture.
Q: 100 MS-NW-S532 80 40 20 25 50 75 100 125 150 m/z Relative Intensity 60
A: Mass Spectra Tells Us about the splitting pattern of molecules.
Q: Give examples of two aspects of the mobile phase a chemist can manipulate to affect the RF of…
A: During chromatography retention factor (Rf) for a chemical substance is calculated as follows: Rf =…
Q: In carbonate mixtures, Vpth > ½Vbcg for an NaOH, N22CO3 mixture. Try to explain why the relationship…
A: We need to explain the relative volume of acid used up in titration of the mentioned mixture of…
Q: CIE standardized instruments of the geometry of 45。 / 0。and d/8。are widely applied for colour…
A: To answer- (a.) With the aid of diagrams, how the measurement is achieved using these two geometries…
Q: The absorption coefficient of O2 in seawater at 0°C is 1.7 mmol/L.What is the concentration of O2 if…
A: Given information, The absorption coefficient of O2 in seawater at 0°C is 1.7 mmol/L.
Q: Give 3 examples for inorganic binders and write their approximate calcination temperatures.
A: A question based on inorganic binders, which is to be accomplished.
Q: What was the average delta t for part 1? From your observations about the experiment, which…
A: Exothermic reactions are those types of reactions in which energy is released in the form of heat…
Q: There is a class of polyesters that have the general structure shown below. For example, in…
A: Since in the above case, molecular weight is constant and it will affect the glass transition…
Q: Amphetamine is a weak base, pKa is 9.9, has a partition coefficient of 50 when distributed between…
A:
Q: The sodium salt of 2-quinizarinsulfonic acid (NaQ) forms a complex with Al" that absorb strongly at…
A: 2-quinazirinsulphonic acid contains two 2 sulphonic acid groups and sodium ions with +1 oxidation…
Q: In an ideal situation, what would the slope of a calibration curve be for an ISE experiment…
A: To determine the slope of a calibration curve be for an ISE experiment measuring K+, NO3-, Sc3+
Q: Biuret Assay accurately quantify protein concentration within the range of 5-150 mg/mL. JUSTIFY THE…
A:
Q: 2 H₂O₂ (ar) -> 2H₂0 (1) + 0₂ (g) 2 Juien data 1202], 11•22×102 | 8.04×10-3 5.30 x 10-3 3-49 x103 tı,…
A:
Q: Propose how the components of a mixture containing Toluene (b.pt= 110.50C) and CCl4 (b.pt. =770C)…
A: The mixture containing the two toluene and CCl4 can be easily separated by the method of fractional…
Q: The following data are obtained from three standard soltions using UV-Vis spectroscopy: What is…
A: Given : Absorbance of solution at 255 nm= 0.180 And molar mass of analyte = 188.65 g/mol
Q: What are the conditions for a successful gravimetric analysis.
A: As per bartleby guidelines I answered only first question so please don't mind.Thanks in advance.
Q: What are the structures of threonine in each part of its titration curve? Give a clear handwritten…
A:
Q: What are the chief differences between the Ninhydrin and Bradford assays for amino acid…
A: Ninhydrin and Bradford assay both are performed to determine amino acids. Nin hydrin reacts with…
Q: How would you expect your plot of Pvap vs. T (°C) to differ if you had examined methanol instead of…
A: Vapor pressure is the measure of how much a substance is prone to go to the vapor state. To compare…
Q: A series of caffeine standards were prepared and scanned with an IR spectrophotometer yielding the…
A: The above graph to determine the percentage of caffeine in aqueous solution of sample and to prepare…
Q: All methyl esters of long-chain aliphatic acids (e.g., methyl tetradecanoate, C13H27COOCH3) show…
A:
Q: The Racah parameter B for [Co(CN)6]3−=460cm−1, and [Co(NH3)6]3+= 615cm−1. Explain the difference in…
A: Given, The Racah parameter (B)) for [Co(CN)6]3−= 460cm−1 and for the compound [Co(NH3)6]3+ =…
Q: what weight of betazole phosphotungstate might be expected in the assay of 0.1550 g sample of…
A:
Q: In an experiment to measure the dipole moment of 1,2 dichlorobenzene, 1.603 g of this substance was…
A: Mole fraction = number of moles of the component / total number of moles of all the components…
Q: Prepare 25, 75 100, 125 and 150 mg/dm3 of Cd(NO3).4H2O in a 500ml
A: Given information, Volume of solution = 500 mL = 0.5 dm3 As 1 mL = 10-3 dm3 Given concentrations :…
Q: Write the significances of Poly Dispersity Index (PDI) and calculate the PDI for given data. Number…
A: Ploy Dispersity Index (PDI) tells us the distribution of molecular weights in a polymer. It is given…
Q: Explain the reactions involved in separation separation of benzoic acid from mixture contain…
A: Extraction It is the separation technique in which the desired compound is extracted using two…
Q: Predict the order of elution of the following compounds from a GC column packed with polyethylene…
A: Gas chromatography The mobile phase used is an inert gas and the stationary phase is gas or liquid.…
Q: Using thin-layer chromatography showed that the black dye used in a brand of writing ink contained…
A:
Q: In general, we want to have absorptions less than or equal to 1. Given that an ethanol solution of…
A: • According to Beer-Lambert law when a monochromatic light having intensity I is passed through the…
Q: Predict relative intensities at m/z 36, 37, and 38 for HCl. Let the intensity of the molecular ion…
A: The relative intensities at m/z 34, 35, 36 for molecule H2S has to be determined.
Q: Variation in volume of extraction solvent: compound X is dissolved in 20.0 mL of solvent B. Assuming…
A: · Volume of Solvent VB = 20.0 mL· Partition coefficient PX·AB = 5.0 · Volumes of Solvent A VA are =…
Q: in the determination of Keq in spectrophotometry, why is it that [KSCN] is lower in the preparation…
A: The equilibrium between Fe3+ and SCN- given as:…
Q: Calculate the concentration of stock tetraoxosulphate 6 acid which has mass density of 2.50 and has…
A: In the given question we have to calculate the stock concentration of Tetraoxosulphate(VI).
Q: Seawater was spiked with phosphate to achieve a quantifiable range of phosphate concentrations by…
A: A question based on tools in analytical chemistry that is to be accomplished.
Step by step
Solved in 2 steps
- Answer the following questions on the basis of the free energies of formation at 25°C shown in the table at the top of the next page: (a) Does the peptide bond between alanine and glycine form spontaneously under standard conditions?The image below is a positive ionization mode Electrospray ionization-mass spectrum (ESI-MS) spectrum for a peptide, obtained using high resolution mass spectrometry. If ionization occurs through de-protonation (assuming that the mass of a proton is 1.0 Da), what is the neutral mass of the peptide, in Da? 627.271 627.604 100 - 627.938 628.271 628.604 628.938 627.0 628.0 629.0 mass/charge (m/z) 628.271 626.271 1878.813 O 1884.813 % AbundanceIf the pH is 2, what is the %HA at of a molecule has a pka = 4? Report the answer to one dpcimal place. Your Answer: Answer units
- I did not understand soultion for the question.. The relationship between Affinity and Association & Dissociation Constant. Four proteins (A-D) all bind the same ligand (X), with different affinities. For protein A & B we know that they have a binding site for X with a Kd (dissociation constant) of 10⁻⁵and 10⁻⁸ M, respectively. For protein C and D we know that they have a binding site for ligand X with a Ka (association constant) of 10³and 10⁵M, respectively. Which protein has the highest affinity for ligand ? Explain your reasoning. How do you make them all in same constant so that values can be compared and the one with highest Ka or lowest Kd (Highest affinity in both cases) can be ruled out.Most of the ultraviolet absorption of proteins at 280 nm is due to their content of a. Glutamate b. Tryptophan c. Alanine d. Aspartatemacollege.instructure.com/courses/352/pages/covid-proteins-lab-manual?module_item_id=D44333 A = abc Where "A" is absorbance, "a" is molar absorptivity, "b" is the path length (length of the cuvette in units of cm), and "c" is concentration (mol/L). The molar absorptivity is an intrinsic property of a molecule which tells us how intensely it will absorb at a particular wavelength. If "A" is a unitless quantity, "b" is in units of cm, and "c" is in units of mol/L, then what are the units of molar absorptivity? Do a little research to learn how alpha-helices and beta-sheets are drawn in protein structures. If most of the absorption properties are coming from the alpha-helices in these proteins, and many alpha-helices have similar absorbance properties across different proteins, which protein would you predict would have the highest molar absorptivity? Your job is to use the following data table to determine the molar absorptivity for each of the following proteins at their respective…
- Shown below is a section of beta-sheet from the protein thioredoxin. Amide nitrogens are indicated by circled Ns, carbonyl oxygens by Os, and hydrogen bonds by gray lines. M strand 1 MO strand 3 (N) O 1 2 3 4 For each strand of the sheet (1, 2, 3, 4) indicate the direction (top or bottom) of the N-terminus. [Select] strand 2 [Select] O [Select] strand 4 [Select] strands 1 and 2 Determine the orientation (parallel or antiparallel) of each adjacent strand to one another. [ Select] AHO strands 2 and 3 [Select] N strands 3 and 4 [Select] >Para-aminobenzoic acid has two ionizable groups. One is a carboxylic acid that ionizes through loss of a proton thereby forming a charged para-aminobenzoic acid carboxylate anion, and the other is an amine connected to an aromatic (benzene) ring that ionizes by accepting an H+ to form a positively charged ammonium ion. The pk of the carboxylic acid is 4.9 and the pk of the amino group is 2.5. Assume that the intracellular pH within a bacterial cell is 7.0. N- H O-H H + H-N- H pK₂ = 2.5 Which of the following structures correctly depicts how most of the para-aminobenzoic acid is ionized at pH 7? A. B. O-H C. H H + H-N- H H D. H N- O-H H pK₂ = 4.9The above amino acid, shown at pH = 7.0 and where every atom (including hydrogens!) is visible, is (D or L) -X-
- The maximum number of amino acid side chains that can potentially form an ionic bond (+lIE- interaction) with the side chain of His is _at pH 21;at pH 7 and_at pH 11.5 andat pH 13.5. (assume no partial charges, only +/- whole number integers) 00: 2; 3:1 01:2:4:1 O2:2;3;0 00:2;3:0 00:2, 4:0 00:0: 2: 2 O none of theseThe pKaa of the conjugate acid of guanidine is 13.6, making it one of the strongest neutral organic bases. Offer an explanation.Draw the most prominent ionization state for phenylalanine (pKa for acidic hydrogens shown below) at pH = 7.0 and pH = 11.0 O NH3 (+) OH pKa -2 pKa -9 phenylalanine