ph Minimum Optimum Maximum Escherichia coli 4.5 7 9 Pseudomonas aureus 4.5 7.5 9.5 Staphylococcus aureus 6.8 Listeria monocytogenes 4.5 7 9.6 Make a discussion on the experiment of the effect of pH on microbial growth.
Q: How many will be present in 15 minutes? * In t minutes there will be At) bacteria present in a…
A: Bacteria are single-cell prokaryotes. They reproduce asexually through cell division. The cells…
Q: Match the chemical reaction to the visual effect of the product/biochemical test description…
A: 1. Assay's colour change relies on intial reduction reaction resulting in a black precipitate if the…
Q: Given the result show below (Lactose Sugar Tube with Durham Tube insert, tube at extreme right) at…
A: Fermentation test is test used to check the potential of microbes to Ferment carbohydrate source .…
Q: Preparation of Microbial Suspension 1. McFarland Standard was not properly shaken prior to use.…
A: Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and…
Q: Amoxicillin/clavulanate potassium (AUGMENTIN) powder for oral suspension is prepared prior to…
A: Amoxicillin-clavulanate potassium for oral suspension is generally used as an antibacterial and…
Q: McConkey Agar has been requested by a hospital that is investigating cases of suspected Salmonella…
A: Mcconkey agar is a media for gram negative bacteria.
Q: 10. You have a 20mM stock solution of biofilm inhibitor compound X. You are asked to prepare a test…
A: In this question, we are given: 1. Concentration of stock solution: 20mM 2. Desired concentration of…
Q: Background and Procedure: The chemical bromothymol blue is an indicator that appears blue in an…
A: Plants use carbon dioxide, water, and light energy to produce glucose in the process called…
Q: Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60%…
A: Menaquinone are commonly known as vitamin K2. The structure of compound indicates that the compound…
Q: Match inhibitors, selective ingredients, pH indicators and other components to correct medium phenol…
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: Determine the minimum inhibitory concentration and the minimum exposure time given the following…
A: MIC is the lowest concentration where we can observe the visible decline in the bacterial population…
Q: aelis-Menten constant an be determined using the Lineweaver-Burk plot and is equal to twice the…
A: Mechaelis-Menten Constant: - Km is used in the enzyme kinetics, it plays very important role in the…
Q: MICROBIOLOGY Help. those are practice questions NOT graded ones! :) --------- is the product…
A: decarboxylase biochemical test is used to distinguish bacteria on their ability to decarboxylate the…
Q: Compute the cfu/g (lactic acid bacteria and coliforms) of burong mustasa samples given the following…
A: The total coliform bacteria test is the simplest basic test for bacterial contamination of a water…
Q: After preparing Vitamin E gel-cream, observed that the finished product texture is gritty and less…
A: Vitamin E is an important vitamin for the reproduction and proper functioning of our body organs,…
Q: ect in ePortfolio idol™M → Caffeine Acetaminophen Solvent front Download 14. A student conducted an…
A: Introduction Amino acids are chemical molecules containing side chains unique to each amino acid as…
Q: The Kirby -Bauer antimicrobial sensitivity testing method relies on: A. The inhibition of one…
A: In Kirby -Bauer antimicrobial sensitivity testing method zone of inhibition is measured to…
Q: Proteus vulgaris is positive for the production of the enzyme phenylalanine whereas Escherichia coli…
A: INTRODUCTION Proteus vulgaris is a Gram-negative, rod-shaped, nitrate-reducing, indole-positive, and…
Q: Lipopolysaccharide (LPS) is a O a. fungal derivative O b. bacterial derivative O. viral derivative…
A: b. bacterial derivative
Q: A solution containing egg albumin (pl=4.6), B-lactoglobulin (pl=5.2), and chymotrypsinogen (pl=9.5)…
A: Column chromatography is a technique used for the separation of solutes present in the sample based…
Q: You are asked to make 10 mL of buffer from a 5X concentrate. invitrogen Te rerder visit…
A: In their working range, buffers resist change in pH. Buffers consist of a weak acid and its…
Q: Oxidase Test: In the document you submit, please describe the results for this test… (like yellow…
A: If organism is oxidase positive then development of purple/ blue color appears in 5-10second. There…
Q: Separation of Amino Acids by Thin Layer Chromatography Lab Questions 4. Why is it necessary to run…
A: Thin Layer Chromatography (TLC) is a type of partition chromatography. Here, partition occurs…
Q: Two of the most important preparation principles to follow when cooking with milk are low heat and:…
A:
Q: Computational True or False. UHT-pasteurized milk sample X which was spread-plated in…
A: Milk offers a favorable atmosphere for micro-organism development, in addition to being a healthy…
Q: Which antimicrobial agent was most effective against your organism? Can you determine from your…
A: NOTE: Since you have posted multiple questions, so we will be solving the first three for you. As…
Q: Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non-…
A: Nutrients are the compounds found in food that give us energy, allowing us to heal and develop,…
Q: S. urens E.coli LOT 1008934 20 22 06 2021-11-29 BIOMERIEUH MHE MHE LOT 1008934920 22 07 2021-11-2…
A: Antibiotic susceptibility test is done to check the sensitivity of a particular bacterium to a…
Q: A microorganism has a D135 of 2 min. How many microorganisms will be left in the food sample after…
A: Antimicrobials are agents that either kill microorganisms (microbicidal) or stop their growth…
Q: Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time…
A: Ampicillin helps in retarding the growth of microorganisms by stopping their growth because it is an…
Q: Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp…
A: Luria-Bertani (LB) broth is a liquid media comprised of several carbon and nitrogen sources. It is…
Q: Discuss the suitability of bromocresol green and thymol blue (2nd change only) for use as indicators…
A: The term acid-base indicator is associated with a dye that plays an important role in changing color…
Q: Inaccurate volume measurements of aliquots during dilution. Effect: Rationale:
A: Introduction For accurate cell counting we need to keep in mind about the dilution factor. As…
Q: Hyrdogen peroxide. Qualitative and quantitative analysis
A: Asked : Qualitative and quantitative analysis of hydrogen peroxide
Q: 7. Why must a pencil be used for drawing the line and spotting? 8. How will the Rf value of a…
A: Thin layer chromatography (TLC) is a chromatography technique that can be used to separate…
Q: As a reference model, a 7 L baffled stirred-tank reactor with a 3:1 (H:D) aspect was employed to…
A: Bioreactors are the vessels that creat a suitable environment for the active conversion of…
Q: Separation of Amino Acids by Thin Layer Chromatography Lab Questions 1. Describe in detail Thin…
A: Chromatography is a technique used to separate mixtures of substances into individual components. It…
Q: Where do you expect to see the highest number of CFU on a plate: Select one: a. When pasteurized…
A: The number of colony forming units on the countable plate is multiplied by 1/FDF to get the number…
Q: Indinavir has a pKa of 5.0, and 10 (A) what is the % dissociation in stomach (pH =8)
A: The nurses administer the drugs to the patient, and thus it is very much necessary for the nurses…
Q: Aerobic degradation of an organic compound by mixed culture of organism in wastewater can be…
A: When oxygen is present, aerobic biodegradation occurs, in which microorganisms break down organic…
Q: 1. TRUE OR FALSE (explain your answers) a) Enrichment medium should not contain inhibitor to allow…
A: Please follow step 2 for detailed explanation.
Q: 6. You were asked by your laboratory instructor to prepare the following: 12 plates (use maximum…
A: The nutrient media contains essential nutrients to support the growth of microorganisms. Different…
Q: Composition of Cell Lysis Buffer
A: We will do calculations to prepare 1 L (1000 mL) of lysis buffer.
Q: Titanium dioxide, which is the most common white pigment in paint, can be produced from the titanium…
A: The ponchon- savarit method diagram for binary distillation allows by one to account for enthalpy…
Q: Requirements of Autoclave for sterilization
A: autoclave is an instrument used for sterilization of equipments and media cultures etc in the…
Q: Graph of Absorbance vs Time (mins) 1.8 1.6 1.4 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time…
A: What is growth rate = it is the number of growth or increase in number of bacteria per unit time…
Q: 14C-labeled cellulose is added to a vial containing some anoxic freshwater lake sediments and sealed…
A: Anoxic freshwater means areas which is depleted of dissolved oxygen. Biochemical oxygen demands…
Q: Using SP-Sepharose as ion exhange resin, indicate the starting and ending pH for the narrowest…
A: Ion exchange chromatography is applicable for the separation of charged molecules. In this…
Q: You have successfully isolated Enzyme 1 (32500 Da [g/mol]) and Enzyme 2 (65000 Da) using size-…
A: Both SDS PAGE and Size Exclusion Chromatography are used for isolation & purification of…
Q: TRUE OR FALSE (explain your answers) a) When grown in XLD agar, Salmonella will increase the pH of…
A: Microbial medium is the support provided to the microorganisms while we grow them in the laboratory.…
Step by step
Solved in 3 steps
- 53. Which one of the following bacteria group CANNOT survive in presence of oxygen? Group of answer choices obligate aerobes obligate fermenters facultative aerobes anaerobes microaerophiles aerotolerant 54. Identify the MISMATCH pair from the following, Group of answer choices E.coli :::: facultative aerobe group Bacteroides :::: obligate anaerobes none of the above is a mismatched pair Chlamydia sps :::: cell culture for cultivation Gardnerella sps :::: bacterial vaginosis Staphylococcus sps :::: can tolerate 20 % salt; cause pimples Rickettsias :::: obligate intracellular parasitehttps://courses.lumenlearning.com/microbiology/chapter/controlling-microbial-growth/ Read this Article about Controlling Microbial Growth and answer the following questions: Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain. What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name at least two factors that can compromise the effectiveness of a disinfecting agent.Explain how bacteria can become resistant to ampicillin. Be sure to mention the following terms: peptidoglycan, beta lactamase, transpeptidase, beta lactam.
- Choose the False statement regarding mechanism of protease? 1. Cysteine proteases perform covalent catalysis, using third group of cysteine as a nucleophile. 2. Serine, threonine, and cysteine proteases all perform covalent catalysis. 3. Metalloproteases and aspartate proteases perform noncovalent catalysis. 4. HIV-1 protease is an aspartate protease and a target of anti-HIV therapies. 5. In metalloproteases, a metal ion cofactor changes the state of oxidation.Escherichia coli O157:H7 is a foodborne pathogen and can cause serious illness in humans by producing toxins that can severely damage the lining of intestines and kidneys. What are the oxygen requirements of coli? What result would you observe in the MTM agar deep test? Explain. Research on the growth requirements of this bacterium is required. What is the role of oxygen and cytochrome c oxidase in aerobic respiration? Why does the nitrate reduction tube turn red after the addition of zinc? Clostridium tetani is a common soil bacterium and causes tetanus. Would you expect tetani to possess the enzyme catalase? Explain. Research on the growth requirements of this bacterium is required.1). SIM Tubes and TSI slants turn black if the organism is able to reduce iron compounds. For which organism(s) below would you expect a black reaction? Circle all that apply. Escherichia coli. Proteus vulgaris. Salmonella typhimurium. Alcaligenes faecalis. Shigella flexneri. 2). If Citrate is a sugar and fermentation generates an acid, why does the BromThymol Blue indicator in the media turn blue and not yellow? 3). Why is Salmonellosis associated with eggs? Can you get Salmonellosis from other animal sources?
- 1. Clostridium butyricum does not appear to have superoxide dismutase (SOD) and catalase enzymes. How would the growth of C. butyricum in oxic conditions compare to its growth in anoxic conditions? On this basis, what is the aerotolerance category of C. butyricum? 2. Citrobacter freundii is a facultatively anaerobic bacterium. Why would you expect this bacterium to grow better (i.e. form larger colonies) in an oxic environment than in an anoxic one? (as a hint, do some research on the “Pasteur effect”)explanation on how bacteria mycobacterium ulcerans should have the ability to be isolated and grown in pure cultureI U Cardo 12 1三 ニ 三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you III
- Which organism can produce pyocyanin? O N. meningitidis O S. pyogenes O P. aeruginosa S. aureus Question 5 Which organism listed below can undergo blebBriefly explain this statement "Antineoplastic natural products- Taxol".please explain at your own words.you grew streak plates of Clostridium sporogenes under both aerobic and anaerobic conditions. Which plates showed bacterial growth, and what does the zone around the metronidazole disc represent? Additionally, explain what types of bacterial growth metronidazole affects and why.
