Paracetamol is a fairly polar molecule while lycopene is not. If you are using a polar mobile phase through a C-18, which compound will have a shorter retention time? lycopene They will not separate and elute at the same time Cannot be determined since the identity of the mobile phase must be known Cannot be determined since it is a C-18 column paracetamol

You run the chromatography system with paper as the stationary phase and 70% isopropanol as the mobile phase. You determine the Rf  of a polar analyte to be 0.40. If you use 100% isopropanol (a less polar solvent) instead of isopropanol as the mobile phase what would you expect to happen to the Rf  value? No value effect on Rf higher  lower could be higher or lower

You would like to calculate the concentration of a protein in a solution measured in a 96 well plate. You have the following information vo in well = 200 μL. pathlength=6 mm, 25000 M1 cm1, Absorbance-2.40. Choose the most correct answer Selected Answer: d. 0.16 mM

29. What exactly is relative retention and retention time? How do you solve problems like this?

A TLC is developed using a mixture of acetone/methanol (both polar solvents) as mobile phase. The solvent front runs 10 cm. Three spots A, B, and C are spotted with the following distances: А: 3.5 сm B: 8 cm C: 5 cm Which spot is the most polar? O A Ов O C O Both B and C

Q2) In the thin layer chromatography experiment, calculate the relative mobility for the two dyes if the distance traveled by the solvent (ethanol) was 3 cm, the distance traveled by the purple dye was 2.5 cm, and 1.5 cm for the red dye? Which dye is considered less soluble in ethanol?

Dr. Waybell designs a study to investigate whether the dose of melatonin taken before bed impacts people's moods. Dr. Waybell rejects Ho and computes 7²=0.05. She has made a Type Il error. O Dr. Waybell retain reject Ho and computes n=0.005. She has made a Type Il error. O Dr. Waybell rejects Ho and computes 7=0.005. She has made a Type Il error. Dr. Waybell retain reject Ho and computes n=0.05. She has made a Type Il error.

Briefly explain how cation exchange chromatography can used in protein separation?

If the stationary phase and the compound being tested are both polar, will the retention factor (Rf) likely be smaller or larger than if a nonpolar compound was tested? Why? 1) The retention factor will likely be smaller as it has a smaller interaction with the stationary phase O 2) The retention factor will likely be greater as it has a smaller interaction with the stationary phase O3) The retention factor will likely be smaller as it has a greater interaction with the stationary phase 4) The retention factor will likely be greater as it has a greater interaction with the stationary phase

During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask.   0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL   1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)