Outline how much of each of these compounds you would weigh out in order to make a 250 mL stock of 500 mM Tris-HCl, pH 8 and a 100 mL stock of 250 mM EDTA. Then, state how much of each of these stock solutions you would use to make a 50 mL solution of TE (also include how much distilled water would be used).

Human Anatomy & Physiology (11th Edition)
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The final step of genomic isolation typically utilizes TE-8 buffer.  To make TE-8, concentrated stock solutions of Tris-HCl and EDTA are made first.

 

Outline how much of each of these compounds you would weigh out in order to make a 250 mL stock of 500 mM Tris-HCl, pH 8 and a 100 mL stock of 250 mM EDTA. Then, state how much of each of these stock solutions you would use to make a 50 mL solution of TE (also include how much distilled water would be used).

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