Order of Transfer of Several Different Bacterial Genes Hfr Strain First Last H thr leu azi ton pro lac gal str met 1 leu thr met str gal lac azi pro ton 2 pro ton azi leu thr met str gal lac 3 lac pro tor azi leu thr met str gal 4 met str gal lac pro ton azi leu thr 5 met thr leu azi ton pro lac gal str met thr leu azi ton pro lac gal str 7 ton azi leu thr met str gal lac pro
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
By conducting conjugation experiments between Hfr and recipient
strains, Wollman and Jacob mapped the order of many bacterial
genes. Throughout the course of their studies, they identified several
different Hfr strains in which the F-factor DNA had been integrated
at different places along the bacterial chromosome. A sample of
their experimental results is shown in the following table:
A. E xplain how these results are consistent with the idea that the
bacterial chromosome is circular.
B. Draw a map of the bacterial chromosome that shows the order
of genes and the locations of the origins of transfer among these
different Hfr strains.
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