Next, you combine double-digested pNUT and double-digested pPOD and then ligate the pieces together (forming a new, intact plasmid when two fragments combine). Among the possible combinations that could result are four new recombinant plasmids, created when either of the pNUT fragments (A or B) ligates with either of the pPOD fragments (C or D). What will be the size of each new plasmid show below? Indicate the correct size in basepairs. Bam HI A+C Sacl bp The A+C plasmid is expected to be The A+D plasmid is expected to be bp. bp.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Need help, please. Please refer to the images attached. The questions are in the second image attached asking for basepair lengths. The first image is just for information.

Next, you combine double-digested pNUT and double-digested pPOD and then
ligate the pieces together (forming a new, intact plasmid when two fragments
combine).
Among the possible combinations that could result are four new recombinant
plasmids, created when either of the pNUT fragments (A or B) ligates with either
of the pPOD fragments (C or D). What will be the size of each new plasmid show
below? Indicate the correct size in basepairs.
Bam HI
Sacl
A + C
bp
The A+C plasmid is expected to be
The A+D plasmid is expected to be
bp.
bp.
Transcribed Image Text:Next, you combine double-digested pNUT and double-digested pPOD and then ligate the pieces together (forming a new, intact plasmid when two fragments combine). Among the possible combinations that could result are four new recombinant plasmids, created when either of the pNUT fragments (A or B) ligates with either of the pPOD fragments (C or D). What will be the size of each new plasmid show below? Indicate the correct size in basepairs. Bam HI Sacl A + C bp The A+C plasmid is expected to be The A+D plasmid is expected to be bp. bp.
The next several questions provide additional practice in predicting and
interpreting the results of restriction digestions. Please use the maps below, for
plasmids pNUT and pPOD, to answer these questions. pNUT is 5,000 bp long,
and pPOD is 6,500 bp long. BamHI, Ncol, Notl, and Sacl are all type II restriction
enzymes; their cut sites on the two plasmids are shown in the maps below.
0, 5000
Bam HI,
4000
Not I
3000
pNUT
5,000 bp
Sac I, 2500
Nco I,
500
Sac I,
5500
0, 6500
pPOD
6,500 bp
Nco I,
1000
Not I,
2000
Bam HI,
3000
Transcribed Image Text:The next several questions provide additional practice in predicting and interpreting the results of restriction digestions. Please use the maps below, for plasmids pNUT and pPOD, to answer these questions. pNUT is 5,000 bp long, and pPOD is 6,500 bp long. BamHI, Ncol, Notl, and Sacl are all type II restriction enzymes; their cut sites on the two plasmids are shown in the maps below. 0, 5000 Bam HI, 4000 Not I 3000 pNUT 5,000 bp Sac I, 2500 Nco I, 500 Sac I, 5500 0, 6500 pPOD 6,500 bp Nco I, 1000 Not I, 2000 Bam HI, 3000
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