Q: Define fluorescent staining.
A: The advent of microscopy started with the development of optical microscopy. Then the level of…
Q: Why can’t nitric acid stains be removed by washing?
A: Nitric acid: it has the chemical formula HNO3. It is a conjugate acid of nitrate and a protic…
Q: Explain the function of water in spore staining.
A: Spores are microscopic units that are accountable for asexual reproduction. Several species of…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: How is xylol used to clean the lens of a microscope? What may happenif xylol is constantly used for…
A: The microscope can be defined as the laboratory instrument which is utilized to examine objects that…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…
A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: Defi ne dyes and describe the basic chemistry behind the process of staining.
A: Staining is a method by which contrast in a sample is enhanced usually at the microscopic level.…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?
A: UV-Vis Spectroscopy It is defined as a quantitative technique that is used to identify the…
Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…
A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…
Q: What are two things that can be done to improve contrast on a microscope?
A: Contrast refers to the darkness of the background relative to the specimen.
Q: plain Why do acidic dyes not stain bacterial cells?
A: Staining is the process of changing the colour of a substance to make it easier to examine tissues,…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What's the theory and conclusion of a acid fast stain?
A: It was Ziehl who originally devised differential staining procedures, that were later refined by…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is safe view dye for? Compare it with Ethidium Bromide.
A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…
Q: What is the purpose of the steam in spore stain?
A: Small tiny objects which are not viewed by the naked eye are called microbes. The microbes are seen…
Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?
A: Cell staining is a technique that is used to better visualize cells and cell components under a…
Q: Classify H and E staining?
A: Hematoxylin and eosin staining (H and E staining) is a widely used histological staining method used…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: Why don't you heatfix a negative stain slide?
A: Negative staining is used to investigate the physical structure, size, and organization of…
Q: What is the significance of putting scale bar in photomicrographs?
A: Scale bar:A scale bar is an instrument or software that attaches to a slide or image when it is…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: Briefly answer the question below: What is the disadvantage of having a really thick smear when…
A: Smear:- It is used to fix the bacteria onto the slide and to prevent the sample from being lost…
Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: What is Ultraviolet (UV) light ?
A: The different kinds of EM radiation that are part of the electromagnetic spectrum, namely: Gamma…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: In the blueprint experiment, what would be the result of not exposing the print to sunlight for a…
A: Introduction Blueprints are created using the cyanotype process, which was invented in 1842 by…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic…
A: Simple staining: * The process staining the bacterial cell with a dye to see bacterial structure…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: do we need to stain microorganisms?
A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
Q: What is the function of staining in light microscopy?
A: The light microscopy or light microscope represents a laboratory instrument required for the…
Q: How do stains used for light microscopy compare withthose used for electron microscopy?
A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…
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- Is it possible to perform double or triple staining using fluorescent dyes? Explain.Select all of the following that represent examples of positive stains. A) nigrosin B) methylene blue C) crystal violet D) India ink E) safraninWhat is the principle of Giemsa staining and components of Giemsa stain?