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Name some variables that would likely affect polarimetry results.
Polarimetry is a laboratory technique used to specify the angle at which polarized light has been pivoted by a given sampling. In biology, polarimetric imaging is useful in the medical field as it can detect abnormalities in cells and tissues such as cancer cells.
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- Why is the accuracy of the calibration curve so critical?What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.
- What is reflectance spectrophotometry What are it's general principles Reflectance spectrophotometry limitation of techniques How to use reflectance spectrophotometry in existing clinical applicationsDo the calibration factor computed varies according to different magnification (objective lenses being used)? Explain.What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.
- Explain why it is not possible to directly measure RV by spirometry.the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.What is the area of field of view in micrometers on 400x magnification. Please show work.
- Identify three factors that would make the reading of a co-oximeter unreliable.The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?Data may be stored optically using a direct view storage tube (DVST).