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- Choose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with waterc: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?
- Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cellsSelect all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixingName a few group of microorganisms where Grams staining will not work and/or not appropriate to use. Why?