Could somone explain exactly what this means below? NOT HW just trying to get a better understanding on what this experiment is about. To produce mice that are deficient for RAC1 in macrophages, female C57BL/6 mice homozygously expressing the floxed Rac1 gene (Rac1fl/fl) [6] were crossbred with male mice homozygously expressing Cre under the monocyte-specific lysozyme M promoter (LC) [12]. Mice were genotyped for Rac1 deficiency as previously described
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Link: Lack of RAC1 in macrophages protects against atherosclerosis - PMC (nih.gov)
Could somone explain exactly what this means below? NOT HW just trying to get a better understanding on what this experiment is about.
To produce mice that are deficient for RAC1 in macrophages, female C57BL/6 mice homozygously expressing the floxed Rac1 gene (Rac1fl/fl) [6] were crossbred with male mice homozygously expressing Cre under the monocyte-specific lysozyme M promoter (LC) [12]. Mice were genotyped for Rac1 deficiency as previously described
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Link: Lack of RAC1 in macrophages protects against atherosclerosis - PMC (nih.gov)
Could you explain the relationship between plaque, macrophages, RAC1, SM22 and smooth muslce cells? Would like to understand this topic below a bit better and the effects these things have on each other.
After discovering that advanced plaques had a greater macrophage RAC1 expression than intermediate plaques, immunofluorescence was used to stain human intermediate (type III) and advanced (type VI) atherosclerotic plaques. The anti-Mac-2 (Cedarlane) antibody was used to identify macrophages, while the RAC1 (Sigma-Aldrich) and SM22 (Abcam) antibodies were used to identify smooth muscle cells.
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