Joshua needs 150mL of 1X TBE buffer. The stock of TBE Buffer is 15X. How much 15X TBE buffer should he use to make 150mL 1X TBE buffer? O 5mL O 25mL O 10mL O 15mL O 20mL O o o O
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- If you want to make 7 L of 1X TGS (Tris/Glycine/SDS Running Buffer) using 8 x TGS stock, how much of the 8 x stock do you need? Report your answer to 1 decimal place.Calculate the amount of BSA you will add to the tube to get the concentrations listed on the chart. Your stock tube is 2mg/mL. The amount of water will be the amount to bring your total volume up to 100uL. Standard Concentration BSA H2O Total volume A 200 ug/mL 100uL B 150 ug/mL 100uL C 100 ug/mL 100uL D 50 ug/mL 100uL E 20 ug/mL 100uL F 10 ug/mL 100uL G 5 ug/mL 100uL H 0 ug/mL 0 uL 100uL 100uLcreate a serial dilution from a 1.5 x 10^8 CFU/ml stock solution that will give you 1500CFU/ml when you plate it.
- I need to calculate the total change in pHp = sqrt(1/8500) = 0.00235 I think this is incorrectYou are given a buffer that is labeled 10x and your instructor asks you to dilute it to 1x in the final tube. If you have a final volume of 100uL in your tube how much of the 10x buffer will you need to add to bring it to 1x? Do you need to add anything else to the tube before using the new solution?
- BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.1 2 3 1 point Mike needs to pipette 25 uL of solution. Which pipette should he use to pipette the most accurate amount of solution? P1000 OP20 1 point You need to pipette a solution of 150 uL. What pipette will you use? What will you set this pipette to (i.e. what will the numbers read on the pipette?) P1000; 0150 2000 P10 P200 ▶⠀ ⠀ > > n P10; 015 1 point Which of the following are best practices for using a micropipette? Select all that apply. Never exceed the upper or lower limit of the pipette If only pipetting a little extra, use a pipette outside of the range limit Point the pipette tip upwards Use a new tip for different liquids When withdrawing liquids, always release the plunger slowly P200; 150 P20; 150You will make 5 dilutions in separate tubes each diluted 1 into 3 in water from the previous tube. Your first tube should be a 1 into 2 dilution from your 100% stock. Each tube should have final volume of 500uL.
- I am confused where the 0.002 came from, and how I could calculate the max volume media.5.5mL convert to uLThe doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show work