In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol The counterstain (safranin) causes the purple color to develop None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The primary stain, crystal violet, is dark purple

In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol The counterstain (safranin) causes the purple color to develop None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The primary stain, crystal violet, is dark purple

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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I U Cardo 12 1三ニ三 rI TI3 4. 111 I. 5. What are the basic shapes of bacterial cells? What are the basic Know the terms. Which types of organisms have axial filaments? How would classify the following in terms of gram stain reaction and shape and arrangement of the cells Escherichia coli? Staphylococcus aureus? Streptococcus pyogenes? Bacillus anthracis? Do these organisms cause any important diseases? arrangements? you III
In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The counterstain (safranin) causes the purple color to develop The primary stain, crystal violet, is dark purple Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol
In the Gram stain, gram-negative bacteria appear red for all the following reasons EXCEPT: They stain with safranin because they are colorless at the step where it is added Safranin is a red stain None of the other four answers (all are true) The peptidoglycan layer in their cell walls is too thin to retain the crystal violet-iodine complex when the decolorizer (alcohol) is added The gram-negative cells' outer lipopolysaccharide layer (outer membrane) prevents their being stained by crystal violet in the first step
21 22 23 24 Short Essay: Answer the prompt in 4 sentances or less. Describe the staining technique used to create the photo. Make sure to describe the structures indicate by the arrow in letter A and the structures circled in letter B. Is this a simple or differential stain? Explain your answer. 1- OB 10 μm
In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purple
All of the following are correctly matched and can be found in bacteria EXCEPT: None of the other four answers (all are correctly matched and can be found in bacteria) Nucleus membrane-bound organelle containing multiple chromosomes Ribosomes - site of protein synthesis Cell wall contains peptidoglycan Cell (plasma) membrane - encloses cytoplasm; phospholipid bilayer with proteins
Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria responsible in any way for the Gram stain reaction?
Would lysozyme or penicillin affect pneumoniae? What factors normally limit the resolving power of a light microscope? In the Gram stain procedure, which step is most time-sensitive?
About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?
What technique is used to stain this bacterium? What kind of stain is used? What type of chromophores does this stain use? What is the purpose of this technique? Do we heat fix when performing this technique?
In Figure 5-5,a. Why do A− and B− cells, by themselves, not formcolonies on the plating medium?b. What genetic event do the purple colonies in themiddle plate represent?
What color would the bacterial cells have when viewed microscopically after the following treatments: Gram Reaction Ammonium Oxalate Gram lodine Ethyl Alcohol Safranin Crystal Violet Gram-positive Gram-negative