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In order to simplify your analysis, rather than including an actual DNA sequence, I have simply included the gene of interest (shown with the blue line) with key regions, including the HindIII you do not need to restriction sites, indicated with labelled arrows. be concerned with the reading frame.

In order to simplify your analysis, rather than including an actual DNA sequence, I have simply included the gene of interest (shown with the blue line) with key regions, including the HindIII you do not need to restriction sites, indicated with labelled arrows. be concerned with the reading frame.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Using the “target DNA” sequence provided in the attached image, what sizes of inserts can be prepared using HindIII digestion? Which one contains the coding sequence?

In order to simplify your analysis, rather than including an actual DNA sequence, I have simply included the gene of interest (shown with the blue line) with key regions, including the HindIII restriction sites, indicated with labelled arrows. you do not need to be concerned with the reading frame. 5' 3' 5' 3' 5' W: Gene of interest HindIll (66) transcription start (1283) translation end (4349) Hindill (4415) translation start (2036) Hindill (2093) transcription end (4962) 3' 5: 3' Hindill (11097). 3' 5
Transcribed Image Text:In order to simplify your analysis, rather than including an actual DNA sequence, I have simply included the gene of interest (shown with the blue line) with key regions, including the HindIII restriction sites, indicated with labelled arrows. you do not need to be concerned with the reading frame. 5' 3' 5' 3' 5' W: Gene of interest HindIll (66) transcription start (1283) translation end (4349) Hindill (4415) translation start (2036) Hindill (2093) transcription end (4962) 3' 5: 3' Hindill (11097). 3' 5
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