In addition to observing similarities to the lac operon, you also notice that this gene is regulated via attenuation, similar to the trp operon. Based on this similarity to this model operon, you could state that ___________. Group of answer choices If a terminator loop forms in the DNA, the expression of the structural genes is halted. The formation of the terminator hairpin followed by a series of Uracil (UUUUUUU) functions similar to Rho-Independent termination to result in the stopping of transcription. The transcription of a leader sequence affects the translation of the structural genes. Never mind – all of these statements are true! Attenuation will be the primary means of transcriptional regulation, with a repressor used as a back up option.

In addition to observing similarities to the lac operon, you also notice that this gene is regulated via attenuation, similar to the trp operon. Based on this similarity to this model operon, you could state that ___________. Group of answer choices If a terminator loop forms in the DNA, the expression of the structural genes is halted. The formation of the terminator hairpin followed by a series of Uracil (UUUUUUU) functions similar to Rho-Independent termination to result in the stopping of transcription. The transcription of a leader sequence affects the translation of the structural genes. Never mind – all of these statements are true! Attenuation will be the primary means of transcriptional regulation, with a repressor used as a back up option.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 11TYU

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When referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, ________. tryptophan inactivates the repressor protein translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels the ribosomes stall during translation of the attenuator region transcription termination at the attenuator region is likely
The lac operon regulates expression of genes required for the breakdown of lactose. Use the schematic diagram of the lac operon to help you answer the following questions about the lac operon. Representation of the Lac operon and upstream regions in E.coli (not drawn to scale!) Indicates 1,000s bases Indicates regions of DNA lacz lacz lacz gene gene gene .... Lac I promoter Lac I gene CRP lac Z lac lac Z lacY lacA binding promoter operator gene gene gene site When wild-type E. coli are grown in media with high lactose and high glucose, which of the following statements are true? RNA polymerase can bind tightly to the lac Z promoter and activate a high level of transcription CRP protein is bound to the CRP binding site There is a basal level of lac Z, Y and A transcription O RNA polymerase can bind tightly to the lac I promoter and activate a high level of transcription
The lac operon regulates expression of genes required for the breakdown of lactose. Use the schematic diagram of the lac operon to help you answer the following questions about the lac operon. Representation of the Lac operon and upstream regions in E.coli (not drawn to scale!) Indicates 1,000s bases Indicates regions of DNA lacz lacz lacz gene gene gene Lac I promoter Lac I gene CRP lac Z lac lac Z lacY lacA binding site promoter operator gene gene gene When wild-type E. coli are grown in media with high lactose and no glucose, which of the following statements are true? V There is a basal level of lac Z, Y and A transcription RNA polymerase can bind tightly to the lac Z promoter and activate a high level of transcription RNA polymerase can bind tightly to the lac I promoter and activate a high level of transcription CRP protein is bound to the CRP binding site
Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in_____ Constitutive expression of the lac operon genes ito sagot ko Lack of expression or reduced expression of the lac operon genes under all circumstances Expression of the genes only when lactose is present Expression of the genes only when lactose is absent
The process in which the two-dimensional structure of RNA from the L region of an operon can either prevent or allow transcription of the structural genes is called _____________. Group of answer choices Activation Co-repression Induction Attenuation Repression
The lac operon regulates expression of genes required for the breakdown of lactose. Use the schematic diagram of the lac operon to help you answer the following questions about the lac operon. Representation of the Lac operon and upstream regions in E.coli (not drawn to scale!) Indicates 1,000s bases Indicates regions of DNA lacz lacz lacz gene gene gene Lac I promoter Lac I gene CRP lacZ lac lac Z lacY lacA binding site promoter operator gene gene gene When wild-type E. coli are grown in media with high lactose and no glucose, which of the following statements are true? There is a basal level of lac Z, Y and A transcription RNA polymerase can bind tightly to the lac Z promoter and activate a high level of transcription RNA polymerase can bind tightly to the lac I promoter and activate a high level of transcription CRP protein is bound to the CRP binding site
A constitutive mutation in the lac operon may be of several types. [Note that constitutive means that the structural genes are always expressed, even if lactose is absent.] Name two types of constitutive mutations. Group of answer choices LacY- and LacI- LacOc and LacZ- LacZ- and LacI- LacY- and LacA- LacI- and LacOc
Use the blanks on the left hand side to put the following statements in order (use numbers or letters to designate the order). All of the statements refer to the diauxic (sequential) growth of a bacterium on the carbon substrates glucose and lactose (see textbook Fig 4.11 on diauxic growth). Remember, the lac operon contains the genes necessary for the lactose catabolism. The glucose substrate is completely consumed, at which time the repressor protein on the lac operon unbinds from the DNA molecule. The lactose substrate is catabolized by the active B-galactosidase enzyme. Glucose, as the preferred carbon substrate, is first consumed by catabolic enzymes that are constitutive (always present). After completion of translation, the B-galactosidase enzyme undergoes folding to form into an active enzyme that can breakdown lactose into its constituent monosaccharides. The messenger RNA code from the lac operon is translated by ribosomal RNAS to form lac-related enzymes, such as…
The symbols a, b, and c in the table below represent theE. coli lac system genes for the repressor (I ), the operator(O) region, and the β-galactosidase (Z), although notnecessarily in that order. Furthermore, the order inwhich the symbols are written in the genotypes is notnecessarily the actual sequence in the lac operon.Activity (+) or inactivity (−) of Z geneGenotype Inducer absent Inducer presenta- b+ c+ + +a+ b+ c- + +a+ b- c- - a+ b- c+/a- b+ c- + +a+ b+ c+/a- b- c- - +a+ b+ c-/a- b- c+ - +a- b+ c+/a+ b- c- + +a. Which symbol (a, b, or c) represents each of the lacgenes I, O, and Z ?b. In the table, a superscript minus sign on a gene symbolmerely indicates a mutant, but some mutant behaviorsin this system are given special mutant designations.Using the conventional gene symbols for the lac operon,designate each genotype in the table.
The diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the MRNA leader region. Suppose a mutant bacteria has region 4 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Lead&r region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA UGGUGG 1 (tryptophan codons) AUG UUUUUU No There is insutficient information to answer the question. O Yes
The diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the mRNA leader region. Suppose a mutant bacteria has region 3 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Leader region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA AUG UGGUGG UUUUUU 1 2 3 (tryptophan codons) There is insufficient information to answer the question. Yes No O O
Lactose permease is encoded by the lacY gene of the lac operon.Suppose a mutation occurred at codon 64 that changed the normalglycine codon into a valine codon. The mutant lactose permease isunable to function. However, a second mutation, which changescodon 50 from an alanine codon to a threonine codon, is able torestore function. Is each of the following terms appropriate or inappropriate to describe this second mutation?A. ReversionB. Intragenic suppressorC. Intergenic suppressorD. Missense mutation