Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: layer
A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…
Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…
A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Why bleach disinfectant will the most effective against probiotic?
A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…
A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…
A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…
Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…
A: Option b
Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…
A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…
Q: Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria.…
A: The bacteria cell envelope is a complex multilayered structure and based on this structure the…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: What would happen if you had skipped the heat fixing step and continued with the staining procedure?
A: There are various staining methods are used for staining the bacteria, namely simple staining,…
Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…
A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…
Q: why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
A: Spores are cellular structures produced by plants, bacteria, and fungi. Spores can be produced for a…
Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…
A: Staining is the method of producing different colours that obtained by the reaction of reagents with…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…
A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…
Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…
A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…
A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: The decolorizer used in the Gram Stain, and the decolorizer used in the Acid Fast Stain, are the…
A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…
Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?
A:
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use…
A: The Gram stain is a differential staining method used for classifying bacteria into either…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…
A: The gram-positive and gram-negative bacteria can be differentiated based on their cell wall elements…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…
A: Gram Staining is the common, important, and most used differential staining techniques in…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would
you expect to see on the slide?
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- Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents? a)Grama's staining procedure b)simple staining procedure c)Acid -fast staining procedure d)Non -acid fast staining procedure e)Scaeffer -Fulton staining procedureIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.
- Can you show the step by step process of Methyl Red test using a schematic diagram?You want to isolate staphylococci from a clinical sample, as you suspect the patient has an infection with Staphylococcus aureus. What media would best isolate staphylococci from the other organisms in the culture?in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
- The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?if an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?
- If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.