I am having trouble understanding and explaining how I should explain this in lamen terms and which sources can be used to help. Report on : Enterobacter aerogenes Part II – Describe the purpose of identifying unknown bacterial cultures in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms. Part III - Methods In this section you are describing in detail all the tests that is done to identify the unknown organism. Gram Stain: Explain differences between gram-positive and Gram-negative organisms. Why One group will end up being purple and another group will be a different color. Very briefly describe the steps of Gram stain. Describe the appearance of your organism under the microscope (shape, color). Tests to identify Gram-negative organisms: List the name of organisms that you are considering. Describe all the tests in the logical and orderly manner. Start by writing the chemical equations; (ALL OF THE CHEMICAL AND BIOCHEMICAL REACTIONS). Make sure to include all of the following information. Accurate name of the test Media that must be inoculated in order to identify your organism Indicate the type of media; selective, differential, enriched, etc. Name of the significant product Color changes that represent positive/negative results will be discussed in the Results Section Include all other relevant information Tests to identify Gram positive organism; List the name of organisms that you are considering. Describe all the tests in the logical and orderly manner. Catalase test is the second test that must be discussed after Gram stain. Make sure to include all the following information as you are describing each test. Accurate name of the test (do not abbreviate) Name of media used to perform the test (do not abbreviate) Is the media selective, differential, enriched, etc. Biochemical reactions (only applicable to catalase test) Name of antibiotic used (if any) Part IV - Results: In this section you will present the results of all the tests that was done to identify your organism (your observation). In the laboratory we tabulated our results and created a chart (ID Matrix). Here you are describing the result of each test. Include the name of Indicator used (if any). Color changes that represent positive/negative results must be mentioned here. Part V - Discussion of Results: This is the most important part of your report. DISCUSS all the results. Discuss in a logical and step by step fashion how you concluded that you had a certain microorganism. You need to explain which observation(s) enabled you to identify your unknown microorganism. To convince your reader you need phrases like; This observation “suggested” that my organism is E. coli This observation “confirmed” that my organism is E. coli Also phrases like; This observation “suggested” that my organism is NOT Enterobacter aerogenes Part VI- Significance of your microorganism Pathogen, resident flora, or opportunist microorganism Diseases caused (if any) Symptoms Treatment Other related information
I am having trouble understanding and explaining how I should explain this in lamen terms and which sources can be used to help.
Report on :
Enterobacter aerogenes
Part II –
Describe the purpose of identifying unknown bacterial cultures in medical
field. Name the step by step experiments/procedures that microbiologists follow in order to
identify microorganisms.
Part III - Methods
In this section you are describing in detail all the tests that is done to identify the unknown
organism.
Explain differences between gram-positive and Gram-negative organisms. Why One group
will end up being purple and another group will be a different color.
Very briefly describe the steps of Gram stain. Describe the appearance of your organism
under the microscope (shape, color).
Tests to identify Gram-negative organisms:
List the name of organisms that you are considering. Describe all the tests in the logical and
orderly manner. Start by writing the chemical equations; (ALL OF THE CHEMICAL AND
BIOCHEMICAL REACTIONS). Make sure to include all of the following information.
Accurate name of the test
Media that must be inoculated in order to identify your organism
Indicate the type of media; selective, differential, enriched, etc.
Name of the significant product
Color changes that represent positive/negative results will be discussed in the
Results Section
Include all other relevant information
Tests to identify Gram positive organism;
List the name of organisms that you are considering. Describe all the tests in the logical and
orderly manner.
Catalase test is the second test that must be discussed after Gram stain. Make sure to include all
the following information as you are describing each test.
Accurate name of the test (do not abbreviate)
Name of media used to perform the test (do not abbreviate)
Is the media selective, differential, enriched, etc.
Biochemical reactions (only applicable to catalase test)
Name of antibiotic used (if any)
Part IV - Results:
In this section you will present the results of all the tests that was done to identify your organism
(your observation).
In the laboratory we tabulated our results and created a chart (ID Matrix). Here you are
describing the result of each test. Include the name of Indicator used (if
any). Color changes that represent positive/negative results must be mentioned here.
Part V - Discussion of Results:
This is the most important part of your report.
DISCUSS all the results. Discuss in a logical and step by step fashion how you concluded that
you had a certain microorganism.
You need to explain which observation(s) enabled you to identify your unknown microorganism.
To convince your reader you need phrases like;
This observation “suggested” that my organism is E. coli
This observation “confirmed” that my organism is E. coli
Also phrases like;
This observation “suggested” that my organism is NOT Enterobacter aerogenes
Part VI- Significance of your microorganism
Pathogen, resident flora, or opportunist microorganism
Diseases caused (if any)
Symptoms
Treatment
Other related information
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