How many grams of sodium acetate trihydrate (m. wt. 136) and how many ml of 1 M acetic acid solution are necessary for preparing 3 liter 0.2 M, pH6.4 acetate buffer? pk, = 4.7

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4.1.3.15. How many grams of sodium acetate trihydrate (m. wt. 136) and how many ml of 1
M acetic acid solution are necessary for preparing 3 liter 0.2 M, pH6.4 acetate
buffer? pk, = 4.7
4.1.3.16. 0.03 g-eq. [H*] is formed during an enzymatic reaction carried out in 0.2 M TrisHCI
buffer (pH=7.8). Calculate the pH at the end of reaction (pK,= 8.1). Calculate the
pH if water were used instead of the buffer! Why is necessary to use the buffer in
this reaction?
Transcribed Image Text:4.1.3.15. How many grams of sodium acetate trihydrate (m. wt. 136) and how many ml of 1 M acetic acid solution are necessary for preparing 3 liter 0.2 M, pH6.4 acetate buffer? pk, = 4.7 4.1.3.16. 0.03 g-eq. [H*] is formed during an enzymatic reaction carried out in 0.2 M TrisHCI buffer (pH=7.8). Calculate the pH at the end of reaction (pK,= 8.1). Calculate the pH if water were used instead of the buffer! Why is necessary to use the buffer in this reaction?
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