Give the use and advantages of the following cultures for the examination of colony characteristics: a. Agar plate b. Agar slant c. Broth cultures
Q: What type of medium is used to maintain and preserve specimens before clinical analysis? a.…
A: Microorganisms are small, unicellular, and microscopic organisms. Any organism capable of causing…
Q: A subculture is a a. colony growing beneath the media surface b. culture made from a contaminant c.…
A: We make subculture from a plate where many different colonies of bacteria are growing.
Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…
A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…
Q: Blood agar media is a _________. Selective media Differential media Rich media Enriched media…
A: A medium can be defined as gel or liquid which helps in growth of a bacterial culture. Medium is…
Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…
A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: List the two main types of culture media and how each differs and the types of microbes which grow…
A: Culture media contains all the elements which the bacteria needs for growth and is not selective in…
Q: 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating…
A: Agar is formed by two components, agarose, and agaropectin molecules. Agar is used as culture media…
Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…
A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…
Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…
A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…
Q: Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes.…
A: The micro organisms are grown in specific containments for identification of the characteristic…
Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…
A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…
Q: Milk is usually pasteurized by a. the high-temperature short-time method c. batch method b.…
A: Pasteurization is the process, in which bacteria are killed and the sterilization is done to improve…
Q: What are the factors which need to be considered in the selection of culture vessel?
A: Culture vessels:- Culture vessels provide a contamination barrier to protect the cultures from the…
Q: Show a concept map about the aseptic technique on Transfer from Broth to Broth Culture, Transfer…
A: Aseptic techniques or sterile techniques are procedures that ensure proper handling and conduction…
Q: A pure culture was inoculated onto a Mueller-Hinton agar plate. The Kirby-Bauer procedure was…
A: The Kirby-Bauer procedure also known as a disk diffusion test is performed to test the antibiotic…
Q: Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri…
A: Following aseptic techniques is an important part while performing an experiment. Aseptic technique…
Q: An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is…
A: An inoculation loop is a tool used by microbiologists to transfer a small culture of microorganisms.…
Q: Using saline tubes containing 9 ml of saline (for dilutions), draw a diagram that would yield…
A: All the bacteria in a colony are identical as they arise from a single bacterium. Thus, a colony is…
Q: C MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow…
A: Microbiology is the branch of science that deals with the study of microorganisms that are too small…
Q: A pure culture is........... A. A culture of three to four different organisms B. A culture of one…
A: Culture in microbiology is spread of microorganisms in a medium of development . Per body tissue or…
Q: differential plate enrichment plate selective plate streak plate pour plate
A: Differential plate: Differential media used to distinguish between two related microbes. It used to…
Q: When viable cell concentrations are too high to count on an agar plate, it is common to use larger…
A: The correct option is shown below.
Q: Features of continuous cell culture include the following, except ________ . a.addition of product…
A: Introduction: A single cell type that may be serially multiplied in culture for a set number of cell…
Q: Define the following terms; streak plate method, and selective media. State the purpose and…
A: Plating is a microbiological method used to separate microorganisms found in a sample. It is used to…
Q: what is the purpose of using oil immersion with the 100x objective lens? a)To prevent refraction of…
A: Answer is option a.) To prevent refraction of light.
Q: A mixed culture is a. the same as a contaminated culture b. one that has been adequately stirred c.…
A: The medium or the growth medium has essential nutrients for the growth of the microbes. There…
Q: Explain shortly the concept of differential centrifugation and how you would limit pellet…
A: Centrifugation is a mechanical technique. It helps to separate mixtures based on their size, shape,…
Q: When is the best time to collect a specimen for bacterial culture? A. acute phase of disease C.…
A: Introduction :- Bacterial growth occurs when a bacterium divides into two daughter cells, a process…
Q: Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking.…
A: Streaking method This method is used to produce isolated colonies of bacteria. This helps in…
Q: Which isolation technique is best used in isolating microorganisms (in countable good plates) from a…
A: The phrase "isolation" in microbiology refers to the separation of a strain from a natural, mixed…
Q: Differentiate between defined and complex culture media
A: To differentiate: Chemically defined and complex culture media
Q: Colony formation can be observed in liquid media. a) Trueb) False
A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…
Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…
A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…
Q: What type of medium is best used to maintain a preserve specimen before clinical analysis? A.…
A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…
Q: Explain the concept of and apply the aseptic technique
A: Bacteria, viruses, and fungi are the microorganism that is present everywhere. Some of them cause…
Q: 1.0ml of a 1/10
A: As it is well known that for calculating original stock of bacteria we use the formula CFU/ml =(No.…
Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…
A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…
Q: How do you observe growth in liquid microbiological media? a) the medium is cloudy or turbid b)…
A: Nutrients are required to grow microorganisms invitro i.e outside the body is called culture medium…
Q: Assuming you are working in a microbiology laboratory and a client sent you a sample for the…
A: Microorganisms or microbes are small living organisms (micrometer-sized) that cannot be visualized…
Q: MSA is a selective medium and contains a higher salt concentration, a pH indicator and Mannitol…
A: Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and…
Q: Give 2 different reasons that scientists might need to isolate a pure culture.
A: Pure culture: a culture of microorganism (usually bacteria) that contain only single type of…
Q: You are testing for Escherichia coli in the sample. What media would you use? and Describe typical…
A: A medium in microbiology is defined as a solid or liquid substance used to culture the…
Q: Make a chart of all the organisms (Columns) with results a. Blood Agar Plate b. PEA Agar Plate…
A: As another way of identifying the effects of temperature on the growth of different organisms,…
Q: Transfer of Culture from Agar Plate to Agar Slant
A: The bacterial colonies of interest, after they are isolated from a particular source, need to be…
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: Elaborate on the advantages of examining colony in an agar plate.
A: An agar plate is a Petri dish which have a development medium set with agar, utilised to culture…
Give the use and advantages of the following cultures for the examination of
colony characteristics:
a. Agar plate
b. Agar slant
c. Broth cultures
Step by step
Solved in 2 steps
- The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolismWhat appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobwrite a detailed description of each of the following tests/media: a. TSI Agar Slant b. SIM Medium c. Urease Test d. The IMViC Tests e. The Catalase Test f. The Oxidase Test
- Which of the following medium would be most appropriate for the bacterial cultures from stomach? a. LB b. Selective C. Differential d. Nutient困 Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following is/are TRUE the use of laboratory instruments and regarding the use laboratory instruments equipment in regards to making of culture media? " and equipment? * Air bubbles on the surface of agar plates Hot plates can be used to sterilize the inoculating loop and needle. can be removed by fanning them with the luminous flame of the bunsen burner. If the agar surface is wet, it can be dried by A drying oven is used to sterilize nutrient agar, a solid culture medium. heating at 30-40 deg C in the drying oven before inoculation is done. The refrigerator is used to grow psychrophiles. All media taken from the refrigerator should be warmed to room temperature before use, P Type here to search 7:11 pm 30/09/2021 ヘロG) ENG 近In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubes
- Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.Using aseptic techniques, list the general procedure of inoculating a broth from a plate culture.For each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E System for Enterobacteriaceae identification. a. time requirement b. specimen preparation c. tests utilized d. anaerobic conditions e. interpretation of results
- Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion methodDescribe the process of qualitative water test for coliform contamination. If shown EMB plates, be able to determine if coliforms are present or not.In an activity about Preparation and Examiniantion of Direct Fecal Smear Provide the following: 1. Objective 2. Materials 3. Procedure 4. Sample drawing Answer 1,2,3,4