From your results, which component had the highest Rf value and which had the lowest?Based on your answer from the previous question, what could be the reason behind some components having an Rf value higher than the others? Compare the qualities of the two components that rendered the highest and lowest Rf After performing the simulation, what do you think are the possible practical applications of chromatographic techniques that are relevant to your program? SPINACH LEAVES EXTRACT COMPONENTSComponentsChlorophyll BChlorophyll AXanthophyllCaroteneDistance1.12.34.54.7travelled fromthe original lineRf value0.220.460.90.94 Paper ChromatographyHELPDistance travelled by thesolvent5Distance travelled byChlorophyll b (dark green)1.1Distance travelled byChlorophyll a (light green)2.3Distance travelled byXanthophyll (yellow)4.5Distance travelled by Carotene(orange)4.7Paper ChromatographyHELPXanthophyll (yellow)4.5Distance travelled by Carotene(orange)4.7Rf value of Chlorophyll b0.22Rf value of Chlorophyll a0.46%3DRf value of Xanthophyll0.9%3DRf value of Carotene0.94 V%3D

Paper chromatography can be used to separate photosynthetic pigments based on their polarity.

From your results, which component had the highest Rf value and which had the lowest? Based on your answer from the previous question, what could be the reason behind some components having an Rf value higher than the others? Compare the qualities of the two components that rendered the highest and lowest Rf After performing the simulation, what do you think are the possible practical applications of chromatographic techniques that are relevant to your program?

From your results, which component had the highest Rf value and which had the lowest? Based on your answer from the previous question, what could be the reason behind some components having an Rf value higher than the others? Compare the qualities of the two components that rendered the highest and lowest Rf After performing the simulation, what do you think are the possible practical applications of chromatographic techniques that are relevant to your program?

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter15: Instrumentation In Medical Imaging

Section: Chapter Questions

Problem 1P

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PART III - Table 3: Absorption Spectrum Data for Spinach Chloroplast Extract Absorbance Wavelength 620 nm 0.147 640 nm 0.058 660 nm 680 nm 700 nm 720 nm Wavelength 380 nm 400 nm 420 nm 440 nm 460 nm 480 nm Absorbance 0.759 0.923 Questions: 1.095 1.075 0.833 10.520 Wavelength 500 nm 520 nm 540 nm 560 nm 580 nm 600 nm 0.054 0.066 0.094 123 Graph: Plot the absorbance data on the graph below. Remember TAILS. Absorbance · 163 ·19 4 •514 · 166 1012 ·003 1. Your graph should have two "peaks." At the highest of the two, what wavelength was most strongly absorbed by the chloroplast pigments? What wavelength corresponds the top of the second peak? What wavelength was least absorbed by the chloroplast pigments? 2. Based on your observations, which wavelength (color) of light might be expected to generate the lowest rate of photosynthesis? 91
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