Explain the rationale behind the separation of different cellular components using centrifuge.
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Explain the rationale behind the separation of different cellular components using centrifuge.
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- In the cell there are three types of filaments that form the cell skeleton. Answer:a)Which they are.b)How they are constructed.c)What their function is.2) Explain how the following cell transport systems work moving materials around inside the cell and other functions. Focus on structure and function, and Include how energy is used to accomplish these movements. A)Tubulin/Kinesin B)Actin/MyosinList and describe the three main types of cytoskeleton. If you wanted to do immunocytochemistry to specifically stain each type of cytoskeleton, what is a protein that could be used for each cytoskeletal type (in other words, what is a unique protein for each cytoskeletal type)? What are three types of actin structures? Describe the involvement of actin structures in cell migration. How is the growth and shrinking of microtubules regulated? Then describe the roles of microtubules in: chromosomal separation during mitosis and the movement of organelles and vesicles within a cell. Describe a possible mechanism on how signaling pathways might impact the cytoskeleton, so that cell migration could be regulated in a localized manner within a multicellular organism. (hint: think about the possible transcriptional regulation of the G-protein regulators) What are 2 main challenges of protein targeting? Then describe one way these challenges are overcome during signal-based targeting and one way…
- Explain the functional significance of cell size and cell shape.Define how to grow cells in culture.12) Match the cell-cell junction on the left with the appropriate function on the right. Note that more than one letter may apply for a particular function and that each letter may be used more than once or not at all. A) Gap junctions provides a very strong attachment point between cells B) Desmosomes allow easy movement of large amounts of substances between adjacent cells C) Plasmodesmata seals the plasma membranes of adjacent cells together, preventing the movement of molecules between them D) Tight junctions
- The properties of integral membrane proteins can be studied by 1)Atomic force microscopy 2)Cryo-sectioning and electron microscopy 3)Freeze-fracture technique and electron microscopy 4)All of the aboveWhich of the following is a function performed by the cytoskeleton?a) provides structural support that determines cell shape and resists deforming forcesb) positions various organelles within the cell interiorc) provides a network of tracks over which materials like mRNA and organelles move within cellsd) serves as a force-generating apparatus that moves cells from one place to anothere) all of these choicesIdentify the structures and functions of microtubule-based cellular structures.
- Name three parameters that need to be stably maintained inside a cell.A) Use the terms/ Phrases in the list provided to NAME the cell components B) Use the terms/ Phrases in the list provided to list the FUNCTION the labeled cell componentUse the analogy of the cell as a manufacturing factory to describe the function of the following cell structures: plasma membrane, mitochondria, nucleus, Golgi, ribosomes, lysosomes, endoplasmic reticulum.