Exit Ticket: Use the diagram below to answer the following questions. DNA Samples 1 2 3 4 5 6 7 Question # Question Answer and Justify 1 This technique used to analyze DNA directly results in (1) synthesizing large fragments of DNA (2) separating DNA fragments on the basis of size (3) producing genetically engineered DNA molecules (4) removing the larger DNA fragments from the samples This laboratory technique is known as (1) gel electrophoresis (2) DNA replication (3) protein synthesis (4) genetie recombination Name one other way besides crime scenes that Gel Electrophoresis can be helpful technology! IIII || |||| ||| 2. 3.

Exit Ticket: Use the diagram below to answer the following questions. DNA Samples 1 2 3 4 5 6 7 Question # Question Answer and Justify 1 This technique used to analyze DNA directly results in (1) synthesizing large fragments of DNA (2) separating DNA fragments on the basis of size (3) producing genetically engineered DNA molecules (4) removing the larger DNA fragments from the samples This laboratory technique is known as (1) gel electrophoresis (2) DNA replication (3) protein synthesis (4) genetie recombination Name one other way besides crime scenes that Gel Electrophoresis can be helpful technology! IIII || |||| ||| 2. 3.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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Directions: Given the DNA strand below. Decode the hidden message in the proteins that will be produce in protein synthesis of the given DNA strand. Identify the (1) complimentary DNA strand, (2) mRNA, (3) tRNA, (4) amino acid sequence, and (5) protein. Use the 1-letter symbol of the amino acids to produced to decode the message.
Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?
Explain Shortly. I need help The emergence of new molecular biology techniques has allowed researchers to determine DNA sequences quickly and efficiently. A) How could knowledge of a DNA sequence be abused? B) How could knowing a DNA sequence be helpful? C) Would you ever consent to having your DNA sequenced. Explain your answer
BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165
5) Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average weight of a deoxynucleotide monophosphate is 328 g/mol. B. You digest a 5 kb plasmid with two enzymes that yield fragments of 4 kb and 1 kb, and resolve the digest on the gel. What would you predict to see in terms of the intensities of the bands?
Paternity Test: Alanna claims John is the father of Timmy, but Leo thinks that he is the father. All of their DNA samples were taken and cut with the same restriction enzyme and run through a gel. 4. Who is the father of Timmy? 5. How many of Timmy's fragments are the same size as his mother's fragments? 6. Whatactually causes the DNA pieces to move through the gel? 7. Describe the difference you see in the gel between John's DNA and Leo's DNA. Alanna's DNA Timmy's DNA John's DNA Leo's DNA
Need to understand how to do this: create the complementary strand of DNA to the DNA strand provided below: 1. ATAGGTCACCGTAA 2. TCCAGA
Answer the following questions related to PCR Bioinformatics for DNA Extraction using Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100 degrees celsius? What would happen if you did not use a chelating agent such as the InstaGene matrix? What is needed from the cells for PCR? What structures must be broken to release the DNA from a cell?
Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…
Procedure: 1. Using the DNA provided transcribe DNA into mRNA. 2. Use the mRNA strand you created and break it up into codons. 3. Plug the codons into the amino acid chart to determine the correct amino acid needed to build that protein. 4. Identify the protein you made by comparing the sequence to the pictures 5. Answer the questions for each protein molecule you build before moving on to the next. Protein 1: DNA A AGACCGTATAC mRNA Amino Acid Sequence 1. Which kind of protein molecule did this gene make? 2 How does this protein help the body maintain homeostasis2
Learning Task 2: Make a model of a DNA template to determine the sequence of bases in th new DNA strands. Then, answer the guide questions that follow. Materials: crayons. Scissors, paste/tape, used folder or illustration board Procedure: Use the pattern of the DNA template: (attached to this LP). Color code, phosphate - blue, deoxyribose sugar = green, nitrogenous base as follows: adenine= yellow, thymine pink, guanine = violet, cytosine = red. And cut the shapes of each nucleotides. Build a model of a strand of a DNA molecule. The strand should contain 6 base" rungs" following the given order of the nucleotides: Guanine, Adenine, Cytosine, Thymine, Cytosine, Guanine. %3D %3D Tape the cutout pattern to form the nucieotides. This will represent the left half of DNA. Make a complementary strand that you made in step 3. Tape the cut -out pattern again forming the nucieotides for the second strand of the DNA molecules. Match the bases of the first strand and the second strand. Do not tape…
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…