Different stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.
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Different stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.
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- Which of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.In histopathology, why do different stains have different affinities for different organisms or different parts of organisms? Explain and include the Hematoxylin and Eosin staining as an example to further understand. Please answer the question concisely and don't just simply give the meaning of Hematoxylin and Eosin staining.List other fluorescent stains. What is the usefulness of each?
- What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.Fluorescence intensities depends on the molecular structure and its environment. Discuss the factors that can affect the fluorescence intensity and explain how the factors increase or decrease the intensity.
- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninGive the color reaction in Wright’s stain and the function of these cells.Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.
- a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.In staining, why is there a need for contrasting stains?