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describe the 3 processes that use moist heat methods specifying time and temperature for each:
pasteurization
autoclaving
tyndallization
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- Which of the following is used in the sterilization of the medium?a) benmargeb) swabc) autoclaved) centrifugee) coolingWhat are the primary uses of the following preparations of culture media? Agar regular slant Agar special slant Agar deep Broth Agar plateOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.
- _____ kills pathogens and some spoilage organisms but does not sterilize substances. _____ kills pathogens and some spoilage organisms but does not sterilize substances. Tyndallization Autoclaving Pasteurization Boilingwhich of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization b. autoclave c. dry heat d. filration e. none of the aboveDescribe the difference between batch and continuous culture techniques
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateIdentify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.Choose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage: a) Drying b) Mineral oil overlay c) Freezing with liquid nitrogen d) Lyophilization e) Periodic transfer to fresh medium
- Distinguish batch culture and continuous cultureWhat microorganisms serve as the reference point in determining the time and temperature of pasteurization?Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%