Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter32: Radiochemical Methods
Section: Chapter Questions
Problem 32.6QAP
Related questions
Question
can you generate the unknown concentration using this information?
Transcribed Image Text:3 Section 9 Lowrys Method Excel Report
4
Absorbance
concentration OD at 720 Corrected
5 (μg/ml)
OD(nm)
(nm)
0
6
7
8
9
10
11
12
13
14 Unknown
15
16
17
BSA
19
88888
60
100
0.237
0.257
0.249
0.247
0.263
0.263
0
0.02
0.012
0.01
0.026
0.026
Raw Data Corrected
0.302 0.065
0.265
0.26
0255
8025
0.245
0.24
0.235
Absorbance OD at 720 (nm)
Determination of unknown BSA
Concentration by Lowry's Method
20
y 0.0002x 0 2422
-0.5819
40
60
NO
BSA concentration (pg/ml)
y 0,0002x+0.2422
0.065-0.0002x 0.2422
100
120
Transcribed Image Text:S.
No
1
2
3
4
5
6
7
BSA
Concentrat
ion
(ug/mL)
0
20
40
60
80
100
To be
Volume
of Stock
BSA (ML)
Blank
Distilled
Water
(mL)
1
.
0.2 200
0.4 00
0.6000
0.6600
0.4 400
0.8 800 02 200
1.01000
0
1.0 10000
0.8 0
Analytical
Reagent
(ml.)
light blue
2.5
2.5
2.5
2.5
2.5
2.5
Incubate
for 10
Temp.
1000, 1000, Boo
2.5
minutes
at Room
Folin's
Reagent
(mL)
0.5
0.5
0.5
0.5
0.5
0.5
0.5
Incuba
te for
37C
minutes
at Room
Temp.
Absorbance
(CD at
50-mm 130
0.331
0-261
0.249
0.263
0.263
0.300
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 3 steps with 3 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning