1 can you complete table 4.7, using Beer's law. whereC= .04 micromoles, e= 18.8850, and b= 1 cmTABLE 4.6ABSORBANCEWAVELENGTHABSORBANCEWAVELENGTH0,6488O.610530.547970.403970.418383853904154104054003950.651670.7080.744480.1S4 520,743180.71471375370365390A 400A-e bc3.Calculation of concentration of p-nitrophenol added (Notenstock concentration 0.2mM):C2MM)(Iml)=C(5mi) 0.04:4 C,= ImlCalculation of t, the molar extinction coefficient:tryCI)C 0004 8850TABLE 4.7TYPE OFBUFFERABSORBANCEpH(A 1(HA]Tris-HClTris-HlphusphatephispmtephusphateLonsphale9.08.0 07027.5 0.5567.0 0.3856.5 0.1926.0RY

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can you complete table 4.7, using Beer's law. where C= .04 micromoles, e= 18.8850, and b= 1 cm

World of Chemistry, 3rd edition

3rd Edition

ISBN:9781133109655

Author:Steven S. Zumdahl, Susan L. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan L. Zumdahl, Donald J. DeCoste

Chapter10: Energy

Section: Chapter Questions

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Question

can you complete table 4.7, using Beer's law. where
C= .04 micromoles, e= 18.8850, and b= 1 cm
TABLE 4.6
ABSORBANCE
WAVELENGTH
ABSORBANCE
WAVELENGTH
0,6488
O.61053
0.54797
0.40397
0.41838
385
390
415
410
405
400
395
0.65167
0.708
0.74448
0.1S4 52
0,74318
0.71471
375
370
365
390
A 400
A-e bc
3.
Calculation of concentration of p-nitrophenol added (Notenstock concentration 0.2mM):
C2MM)(Iml)=C(5mi) 0.04:4 C,= Iml
Calculation of t, the molar extinction coefficient:
try
CI)C 0004 8850
TABLE 4.7
TYPE OF
BUFFER
ABSORBANCE
pH
(A 1
(HA]
Tris-HCl
Tris-Hl
phusphate
phispmte
phusphate
Lonsphale
9.0
8.0 0702
7.5 0.556
7.0 0.385
6.5 0.192
6.0
RY

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