Can you classify household disinfectants as sterilants? What organisms (if any) survive after the use of common household disinfectants that would not survive the use of sterilization/aseptic technique? List a minimum of two organisms that would survive.
Q: How would you use a differential and selective medium in the microbiological analysis of a…
A: Differential media refers to the media that allows for the visual identification of various…
Q: Suppose you are checking the microbial quality of a raw material using agar culture media. After…
A: Unwanted microbial growth occurs when no proper care is taken while tranferring the culture on the…
Q: Define sterilization in terms of microbiology.
A: Sterilization is a process by which all the possible living forms like bacteria, fungi, protozoa and…
Q: Why have scientists developed numerous methods to classify microorganisms?
A: Microorganisms are very diverse. They include bacteria, fungi, algae, protozoa. The identification…
Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?
A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…
Q: Why is it necessary to maintain aseptic conditions in microbiology?
A: Aseptic conditions means microbs free environment. Microbs means bacteria, virus and fungi. It may…
Q: Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What…
A: There are two main microbiology methods used for isolating bacteria from a clinical sample in order…
Q: What is the importance of agar plates and bacteria cultures in food microbiology?
A: There are different culturing techniques such as agar plates, broth culture, stab culture and solid…
Q: Describe the term resistance and biofilm related to microbiology.
A: The ecosystem is composed of species such as plants, animals, and microbes. Microorganisms, which…
Q: Explain the difference between sterilization, disinfection, antisepsis, pasteurization? Give…
A: Microorganisms are present everywhere. They are not visible with naked eyes but are present at…
Q: Why is it important to become familiar with the microbiology laboratory's apparatus and equipment?
A: You should always know your lab equipment well before you do any type of experiment because without…
Q: The following data were obtained by incubating gram-positive bacteria in nutrient medium +…
A: Gram-positive bacteria are bacteria that test positive in the Gram stain test, which has…
Q: A microbe is growing on Sabouraud dextrose agar, which has a slightly acidic pH. Which of the…
A: A culture medium contains enough nutrients for the growth of targeted or desired microbe. Different…
Q: Summarize the terms used to characterize microbes based on they physical growth factors including...…
A:
Q: A culture of bacteria is found to have a DRT of 2 minutes. How long would it take to reduce the…
A: Heat is used for sterilization of those substances that can withstand it over longer periods or…
Q: Describe the step by step method of preparing culture media in microbiology.
A: Culture media are the media or the substrate used to grow microorganisms. There are different types…
Q: among these five areas such as: air, faucet, doorknow, table, and floor, in the laboratory is…
A: Microorganisms are present everywhere in nature, they are ubiquitous in their dwelling nature ie…
Q: Describe and give the principle of each equipment used for sterilization in microbiology.
A: Sterilization is the method of removal, killing or inhibition of all forms of lives, spores or…
Q: Compare and contrast moist and dry heat methods of microbe control and give 2 examples of each.
A: The growth of microbes in an inanimate object can lead to the contamination of the particular…
Q: If you had a mixed culture that contained a Gram-negative enteric bacterium and Gram-positive…
A: MSA or mannitol sale agar used in microbiology. It encourages growth of some group of bacteria…
Q: What is the importance of using a test/control organism in describing the cultural characteristics…
A: The food materials on which the organism is developed is called culture medium and the development…
Q: What is the principle behind sterilizing media before use and before disposal
A: Microorganisms may be beneficial or harmful. The harmful microorganism causes many infections which…
Q: The following will prevent the growth of bacteria: Keeping food at room temperatureKeeping food…
A: Introduction Bacteria Are Single-celled Organisms That Do Not Belong To Either The Plant Or Animal…
Q: Why is it critical to be able to determine the overall number of bacteria present in a food sample?
A: Hi, Thanks For Your Question. Answer : Monitoring Of Total Bacterial Count Is Necessary, Because…
Q: Why are Sterilization, Antisepsis and disinfection necessary?
A: Sterilization is the process of killing or deactivating all forms of microorganisms and spores.…
Q: During the fall 2001 bioterrorist attack in which anthrax endospores were sent through the mail, one…
A: Introduction: Spores are the extremely resistant resting-state that are made at the time of…
Q: How we can isolate microorganism from food? Please briefly explain at your own words.
A: Microorganisms are cosmopolitan in nature, which means they are present everywhere- in air, on…
Q: Why must water be subjected to microbiological analysis?
A: Water is used for various purposes in daily lifestyle including food. Thus, it should be monitored…
Q: Please label the figure to demonstrate your understanding of selective and differential media.…
A: A culture media or growth media is a liquid, gel or solid designed that support the growth of…
Q: Define the following (Microbiology) Lag phase Exponential phase Stationary phase Death phase
A: Bacteria are prokaryotic organisms. They undergo asexual reproduction that occurs commonly by the…
Q: What is the difference between culture media and cultural environment? Are environmental conditions…
A: Microbiology is the field of biological science that deals with the culture, growth, identification…
Q: What compounds or chemicals might be added to a medium to make it selective?
A: Culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth of…
Q: What other fields of science besides microbiology is the aseptic technique used?
A: Aseptic technique refers to the procedures that prevent the contamination of microorganisms. They…
Q: why they use less bacteria in industrial use than recreational use of water? Industrial- (< 100 /…
A: The bacteria are often used for cleaning water. Bacteria are widely used for a number of water…
Q: Which of the following control agents help to be used to achieve sterility? - Virucide - Sporocide…
A: Sterilization can be achieved through various means, including heat, chemicals, irradiation, high…
Q: Among these five areas in the laboratory: air, faucet, table, floor, table, the doorknob, which is…
A: Ans: Microorganisms: The microscopic, unicellular organisms are referred to as microorganisms. They…
Q: Based off Pasteur’s sterility experiment, which of the following choices is least likely to result…
A: Louis Pasteur was the scientist who invented pasteurization, which is a technique to kill…
Q: Is there any pH buffering system or device for acidic microbiological experiments?
A: All biological processes are dependent on pH, cells and organisms must maintain their specific and…
Q: Based on the results of this Gram stain and the ID matrix below, which of the following microbes…
A: Gram' s staining helps in bacterial identification and their further classification. On the basis…
Q: Is the lyophilization process a suitable method for the preservation of all microorganisms
A: preservation of bacterial culture is an important step in microbiology . there are several methods…
Q: What are the different Microbiological sterilization techniques used for Microbiology equipment and…
A: Microbiology is the branch of biology that deals with the study of microorganisms such as bacteria,…
Q: What are the conflicts over spontaneous generation of microorganisms? Describe the golden age of…
A: Answer: Introduction: Spontaneous generation is the term meaning the usual creation of living…
Q: What are the advantages and disadvantages of plate count technique over other methods of quantifying…
A: Plate count is a technique in which bacteria are counted by growing on the agar plates. Total…
Q: MacConkey lactose agar is a medium that kills all microbes except for gram-negative bacteria, and it…
A: Agar is a galactose-based heterogenous polysaccharide obtained from seaweed(red algae). It is…
Q: What is the difference between disinfection, antisepsis, and sterilization?
A: Microorganisms are present every where. They are not visible with naked eyes but are present at…
Step by step
Solved in 2 steps
- For each item on the following list, propose a reasonable method ofsterilization. You cannot use the same method more than three timesfor the whole list. The method must sterilize, not just disinfect; and themethod must not destroy the item or render it useless unless there is noother choice. After considering a workable method, think of a methodthat would not work. Note: Where an object containing something isgiven, you must sterilize everything (for example, both the jar and thepetroleum jelly [Vaseline] in it). Some examples of sterilizing methodsare autoclave, ethylene oxide gas, dry oven, and ionizing radiation.room airseruma pot of soilplastic Petri dishesheat-sensitive drugscloth dressingsleather shoes from a thrift shopa cheese sandwicha flask of nutrient agaran entire room (walls, floor,etc.) rubber glovesdisposable syringescarcasses of cows with “madcow” diseaseinside of a refrigeratorwinea jar of Vaselinefruit in plastic bagsmilkorchid seedsmetal instrumentsmail contaminated…Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.List two methods of high level disinfection.
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperYou are hired in one of the microbiology labs in Memphis and your first assignment to identify microbial samples from a local restaurant. You are given a set of slides and asked to classify the specimens as Gram positive or Gram negative. 6) What would be the last reagent you use to identify the bacteria? 7) What type of visual aid you need to help you see the bacterial samples clearly? 8) After the use of what reagent, you will be able to decide if you have a Gram positive or Gram negative bacteria? **Please give the answer**From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:What are the approaches for sterilization during the production of sterile products? Please answer at your own wordsCONTRAST the following pairs of terms: Sterilize and Disinfect Sanitization and Antisepsis Bactericide and Bacteriostatic
- Explain why it is important to use only a small amount of bacteria when preparing a smearMake a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time MaterialsDefine the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?