Which conditions would you use if yoOu wished to isolate protein d from the cell membrane? lonic interactions are often used to keep proteins like b and d together.
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Q: Which conditions would you use if you wished to isolate protein d from the cell membrane? Ionic…
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- Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.In size exclusion chromatography, which component will elute last in the set up? Group of answer choices Large proteins will elute last because they pass through the pores of the gel beads. Small proteins will elute last because they do not pass through the pores of the gel beads. Large proteins will elute last because they do not pass through the pores of the gel beads. Small proteins will elute last because they pass through the pores of the gel beads.in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Main type of centrifuge *Subtype centrifuge * Speed range *Operational temperature range*Type of Centrifugation
- You have a suspension of cells at a concentration of 5 x 106 cells/mL in culture media. The protocol you’re using for your experiment asks you to dilute the cells 1:10 in fresh culture medium to make a 20 mL suspension. How much of your original suspension and fresh culture media will you need? What’s the concentration of cells now in your working suspension?Explain the differences between gel electrophoresis and column chromatography. Address the principles behind each separation. Why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?A powdered complex medium can be stored for months in the lab. However, after rehydrating the medium it must be used immediately. Why?
- Why is it important to be able to accurately measure protein content in a sample? For example, by using different methods such as colorimetric assays and UV assays.In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer. In addition to H2S production and motility, for what other test can SIM medium be used?
- TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?You are planning an experiment to treat cells in culture with compound A at a final concentration of 10 µM. The stock solution concentration of compound A is 1 mM. What two controls should you have in your cell culture experiment using compound A? Explain both the type of controls and the concentration or volume of the treatments.In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?