) Below are some events that occur in the process of translating mRNA into a protein in a bacterial cell. Pick the best order of events. EF-G–GTP binds to the tRNA in the A site and causes the tRNA to advance to the P site. - The rRNA in the ribosomal 30S subunit base-pairs with the Shine-Dalgarno sequence in the mRNA. - Peptide bond forms between the growing amino acid chain in the P site and the new amino acid in the A site. - IF-3 binds in the E site. - RRF binds in the A site. - RF-1 or -2 binds in the A site. - Ribosomal complex dissociates. - Peptide chain dissociates from final tRNA. - fMET tRNA binds to the start codon in the mRNA. - RF-3–GTP causes displacement of RF-1 or -2 and its exit from the ribosome. - EF-G–GTP binds to the A site and causes RRF to shift to the P site. - A conformational change in the 30S subunit allows the 50S subunit to bind, forming the 70S ribosome.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
1) Below are some events that occur in the process of translating mRNA into a protein in a bacterial cell. Pick the best order of events.
EF-G–GTP binds to the tRNA in the A site and causes the tRNA to advance to the P site.
- The rRNA in the ribosomal 30S subunit base-pairs with the Shine-Dalgarno sequence in the mRNA.
- Peptide bond forms between the growing amino acid chain in the P site and the new amino acid in the A site.
- IF-3 binds in the E site.
- RRF binds in the A site.
- RF-1 or -2 binds in the A site.
- Ribosomal complex dissociates.
- Peptide chain dissociates from final tRNA.
- fMET tRNA binds to the start codon in the mRNA.
- RF-3–GTP causes displacement of RF-1 or -2 and its exit from the ribosome.
- EF-G–GTP binds to the A site and causes RRF to shift to the P site.
- A conformational change in the 30S subunit allows the 50S subunit to bind, forming the 70S ribosome.
Step 1, 2, 3, 4 ,5 ,6 , 7, 8, 9, 10, 11, 12
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