Be sure to answer all parts. Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K = 5.01 × 10 and pK = 8.3) (a) Calculate the pH of the Tris buffer after mixing 11.0 mL of 0.20 M HCl solution with 25.0 mL of 0.10 M Tris. (b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00019 mol of H* was produced. What is the pH of the buffer at the end of the reaction? (c) What would be the final pH if no buffer were present?

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Chapter16: Acid-base Equilibria
Section: Chapter Questions
Problem 16.123QP: A 25.0-mL sample of hydroxylamine is titrated to the equivalence point with 35.8 mL of 0.150 M HCl....
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Be sure to answer all parts.
Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems.
(K = 5.01 × 10 and pK = 8.3)
(a) Calculate the pH of the Tris buffer after mixing 11.0 mL of 0.20 M HCl solution with 25.0 mL of
0.10 M Tris.
(b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00019
mol of H* was produced. What is the pH of the buffer at the end of the reaction?
(c) What would be the final pH if no buffer were present?
Transcribed Image Text:Be sure to answer all parts. Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K = 5.01 × 10 and pK = 8.3) (a) Calculate the pH of the Tris buffer after mixing 11.0 mL of 0.20 M HCl solution with 25.0 mL of 0.10 M Tris. (b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00019 mol of H* was produced. What is the pH of the buffer at the end of the reaction? (c) What would be the final pH if no buffer were present?
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