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- An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc should be 1x and answer shoud be in ulAn AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc of the loading buffer should be 1x and answer shoud be in ulIf the DNA was replicated using the dispersive model, what would you have expected to observe in the generation 2 sample? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b с d e Question 6 Homework Answered 6.0 g 3 distinct bands (one 15N, one 14/15N, and one 14N) 2 distinct bands (one 15N and one 14N) 2 distinct bands (one 15N and one 14/15 hybrid) Only one distinct band (15N) f Only one distinct band (14N) Only one distinct band (14/15 hybrid) No bands
- What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units. Sample bp 6000 5000 2000 1000 500 250 100 50 20 M 2.35u 4.26u 5.47u 6.67u 7.88u 9.47u 10.68u 12.27u 2.67u 10.26u Dye front: 16uThe sample furthest to the left contains the DNA size standards. What is the purpose of this sample? * Negative End + #4 Positive EndIn this gel, if lane two is DNA from the crime scene, which lane contains DNA from a suspect matching the crime scene? If the numbers loaded too spaced out then count across left to right. 2 5 6 B C O Lane 3 O Lane 4 O Lane 5 O Lane 6 O None of the lanes
- Assuming you are working with the DNA from a single organism, in DNA gel electrophoresis the different bands in the final gel result because the DNA molecules ________. are from different organisms have different lengths have different nucleotide compositions have different genesWhy is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…O 29% 48% 52% AKS 5a: Which of the following combinations is true of the nucleotide composition of a sample of DNA? * O A = C O A = C AND C = T O C+T= A+ G O G+C = T+ A Back Next
- What was the first, DNA or RNA? Justify your answer with solid reasonBamHI KpnI SpeI XhoI PatI HindIII 400 500 200 300 700 NotI 75 2580 ECORI Frog DNA BamHI 575 KpnI HindIII 625 2150 HindIII 700 PstI clal 750 РКАВОО 2700bp 915 1900 SpeI AluI 1050 1525 BamHI ori You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp' and tet and you want to use enzymes that cut within the insertional marker gene. (Note that tet means that there is no functional tet gene in the plasmid.) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone segment of frog DNA The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: Oampicillin only Otetracycline…QUE Marker CS1 CS2 J DNA1 DNA1 DNA2 DNA2 Enz 1 Enz2 Enz1 Enz2 [[ 1 1 11 JI Marker TE HC (CC Compare the two suspects DNA Samples from tubes 4 and 3 with the crime scene samples CS1 these were all cut with Restriction enzyme 1) Then compare the suspects DNA from tubes 2 and 4 with the crime scene sample CS2 (all cut with Restriction enzyme 2) om AUT Figure 7.2 Example gel image of suspect and crime scene samples. Samples were digested with restriction enzymes and run on a 0.8% gel. Please note that the markers used in this experiment may not match the markers in this figure.