AUTOIMMUNE DISEASE ASSAYS ANA (Anti-Nuclear Antibody) What other methods are used to test for presence of ANAs? Give the principle of each. How does each of the listed methods differ from the one performed in the lab?
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AUTOIMMUNE DISEASE ASSAYS ANA (Anti-Nuclear Antibody)
- What other methods are used to test for presence of ANAs? Give the principle of each.
- How does each of the listed methods differ from the one performed in the lab?
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- AUTOIMMUNE DISEASE ASSAYS ANA (AntiNuclear Antibody) Why are ANAs produced by the body? How are these ANAs produced by the body? Why is the homogeneous pattern most common in SLE?There are many immunotherapeutics now being used to immunomodulate recipients that are either fully human monoclonal antibodies or partially humanized monoclonal antibodies. Discuss whether or not they may interfere with solid phase antibody assays, CDC assays or cell based flow cytometry crossmatches.Explain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity and robustness when compared to other test methods? Please discuss refering to original articles as examples Covid 19.
- 1. What is Du? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.7 2. Why do we need test for Du when weak or no reaction is obtained in Rh typing? 3. What is required to demonstrate the presence of cells carrying a weak D antigen? 4. Explain the mechanism behind the existence of Dº. 5. Cite possible situations in which an attending physician would request for an emergency screening for the presence of "D" antigen.1. What is Du? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.7 2. Why do we need test for Du when weak or no reaction is obtained in Rh typing? 3. What is required to demonstrate the presence of cells carrying a weak D antigen? 4. Explain the mechanism behind the existence of Du. 5. Cite possible situations in which an attending physician would request for an emergency screening for the presence of "D" antigen.1. What is an antiserum? 2. What are the potency requirements in an antiserum? ANSWERS TO QUESTIONS ON LABORATORY ASSAY NO.4 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B
- describe the indirect fluorscent antibody techniqueRheumatoid Factor RF Latex Test RF is a rapid latex agglutination test kit for the detection of rheumatoid factor (RF) in human serum. RF is found in sera of patients with rheumatoid arthritis and is believed to be IgM antibodies directed against the patients own immunoglobulin G. Principle: The RF latex particles are coated with specially purified human gamma globulin. When the latex suspension is mixed with serum containing elevated RF levels on a slide, clear agglutination is seen within 2 minutes. Questions 1. Is Rheumatoid Arthritis an autoimmune disease? 2. Differentiate the different types of Immunoglobulins 3. Differentiate Rheumatoid Arthritis from Gouty ArthritisQuestion. If you are working in a diagnostic lab, what will be your choice among Monoclonal or Polyclonal Antibodies method for detection of specific pathogen? Give sufficient justification for selection as well as rejection of any given methods.
- Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingIf you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidineOutline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.