An enzyme preparation converts 5 μmol of substrate into product over a period of 0.5 min. This mixture contains ...
Q: A researcher has measured the initial rate of an enzyme-catalyzed reaction as a function of…
A: Km and Vmax are important features of an enzyme thatvcan determine the efficiency of the enzyme.…
Q: A water (H20) molecules is
A: Water occurs as a liquid on the Earth's surface under normal conditions, making it invaluable for…
Q: Given the GC chromatogram below: 500000 400000 100000 10.767 21.62622 977 13.644 12.343 17938 200000…
A: In GC-MS (Gas Chromatography -Mass Spectroscopy ) analysis, the plant extract will first be…
Q: Consider a yeast cell undergoing fermentation but with defective alcohol dehydrogenase (hint: In…
A: The oxidation-reduction reaction is also called the Redox reaction. This reaction involves the…
Q: Name of the vitamin source of CoASH A. B12 B. B3 C. B9 D.B1 E. B7
A: Some enzymes are dependent on cofactors for their enzymatic activity. There are two types of…
Q: Match the following methods of Analysis: Lipid anchor is myristic acid and linked to a glycine…
A: Lipids are complex fatty acids, is in storage form and has many biological functions.
Q: What are the monomers that make up nucleic acids? Explain.
A: Amongst the biological macromolecules, the DNA and RNA falls under the category of nucleic acids.…
Q: Carnitine: A fat mover. Explain the mechanism of action of carnitine in losing weight? (Lipid…
A: The chemical name of carnitine is β-hydroxy-γ-trimethylammonium butyrate. It is a hydrophilic…
Q: What TYPE of inhibition is observed in the following plot: 1/v₂ Intersect at Y axis 1/Km Competitive…
A: Enzymes can be inhibited , when the substrate and inhibitor compete for the binding to the same…
Q: Enzyme Inhibitors inhibit the activity of the enzyme. i) Competitive Inhibitors: ii) Non-Competitive…
A: Enzyme inhibitors: Many substances can prevent enzymes from catalyzing or substantially diminish…
Q: ATP ACCOUNTING, Provide what is being asked for. Show all relevant calculations and summarize your…
A: Hydrolization of lactose gives the galactose and glucose moieties. Galactose are converted to…
Q: Match these drugs with the following functions Cell wall synthesis inhibitors 50s Ribosomal…
A: The antibiotics are compounds that act by selectively targeting crucial cellular pathways in…
Q: High amount of citrate decreases the activity of fructose 1,6-bisphosphatase. True or False
A: Fructose 1,6-bisphosphatse enzyme participates in gluconeogenesis and gluconeogenesis is a pathway…
Q: Define and simplify each of the words meaning in your own words. amino acid transporter:…
A: Introduction: Amino acids are molecules that contain an amine group, a carboxylic acid group, and a…
Q: What are the applications of human genome into health, physiology, and biochemistry?
A: The Human Genome Project is a large-scale scientific project aimed at decoding the chemical makeup…
Q: KM is determined by measuring the reaction velocity of two enzymes (X and Y) at different…
A: Enzymes are usually protein molecules which catalyzes a biochemical reaction by decreasing its…
Q: Write an analysis of the results, interpreting what you see in the smaller gell focusing on 6A, 6B,…
A: DNA fingerprinting is a technique which enables us to identify an individual via his unique Genome…
Q: Please state if the statements are true or false. 1. An l-sugar is a sugar where the -OH group at…
A: Sugars are also called carbohydrates. Carbohydrates are composed of carbon, hydrogen, and oxygen in…
Q: Refer to the diagram of the Krebs cycle Which substance could be considered as the most “heavily…
A: The Krebs cycle is an amphibolic pathway, which is the central hub of metabolism and synthesizes the…
Q: Is there more than one subunit shown? Is your protein bound to anything else? Describe the…
A: Defensins are proteins that play defense role against bacteria and viruses. They are cysteine rich…
Q: One of the blocking solutions that is traditionally used is non-fat milk, but this is not…
A: A phosphoprotein is a protein that has a single phosphate group attached to it, or a complex…
Q: Which of the following statements is TRUE about fatty acid activation before ß-oxidation? O The…
A: β-oxidation is the process of breakdown of fatty acids, in order to release energy during the…
Q: The number of CO2 molecules liberated for each molecule of acetyl-CoA during oxidative…
A: Tricarboxylic acid (TCA) or Krebs cycle is the oxidative or aerobic part of respiration. This cycle…
Q: Explain how osmosis works including the concept of concentration gradient
A: Osmosis is a vital process in many biological systems. For example, it is responsible for the…
Q: he amino acid alanine, shown below, contains all of the following functional groups except A. an…
A: Amino acids are the building blocks of proteins which are composed of amino group (NH3+), carboxyl…
Q: Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or…
A: Thin-layer chromatography is a technique that is used for the separation and identification of…
Q: Consider a 17:0 fatty acid in a mammalian cell where propionyl CoA is completely oxidized. For each…
A: β-oxidation of fatty acid occurs in mitochondria. Before β- oxidation, activation of fatty…
Q: What is/are the functions of the following: a. Lecithin Granules b. Hyaline Bipolar Granules c.…
A: Introduction: Lecithin is a fatty substance present in plant and animal tissues. It is important…
Q: Calculate the unknown concentration of the PROTEIN C with an absorbance value of A412 given the…
A: Protein concentration can be determined with the help of spectroscopy. Spectrophotometer is an…
Q: Y [S] Fig. 12.2 Graph of Y against [S], at fixed protein concentration, where the binding shows…
A: Allosteric regulation is a mode of regulation of an enzyme or other protein. Allosteric activators…
Q: Chlorophyll has iron as the metal center of the porphyrin ring. True False
A:
Q: briefly explain the importance of the degeneracy of the genetic code in the translation process.
A: The genetic code can be defined as a collection of rules that living cells use to translate…
Q: 1. For one mole of the fatty acid residue, determine the following:
A: The transferring of electrons from electrons Carrier and finally accepting by the Oxygen. The…
Q: Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0).…
A: Behenic acid is a saturated fatty acid with 22 carbon atoms and possesses the general formula,…
Q: A radiolabeled glucose solution is utilized as a nutrient source for a human myocyte in order to…
A: Glucose is the most favored respiratory substrate for most forms of life and myocytes (muscle cells)…
Q: A heptapeptide was analyzed by Pinky. She reacted it with carboxypeptidase and found out that it…
A: A polypeptide is a sequence of amino acids that are linked to each other via peptide bonds. The bond…
Q: 45. find the connection among the words below and choose the letter of the word which is different…
A: Gel electrophoresis is a technique used to identify, quantify, and separate nucleic acids. The…
Q: 1. How are organic molecules related to all living things?Explain. 2. Draw an Illustration showing…
A: One or more cells make up every living thing. Living things react to their surroundings and stimuli.…
Q: Calculate the unknown concentration of the PROTEIN B with an absorbance value of A358 given the…
A: Tp calculate the unknown concentration of protein B from absorbance value, we first need to plot a…
Q: Are tRNAfmet and tRNAmet made from the same aminoacyl tRNA synthetase?
A: Amino acyl tRNA synthase pairs tRNA with amino acid that their anti codon codes for. Amino acyl tRNA…
Q: II. Provide the chemical formula for each of the following compounds. 1. sodium sulfate 2. sodium…
A: Chemical formulae are a way to represent any chemical compound using the symbols for the components…
Q: Chymotrypsin, trypsin, and elastase are digestive enzymes called scrine proteases. The serine…
A: Aminoacids are classified based on the nature of their side chain. They are Acidic aminoacids :…
Q: No plagiarism please. Use your own words. Thanks. Discuss the preferred locations of different…
A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: what is the inhibitor of Acetyl-Co
A: Fatty acid metabolism includes Fatty acid biosynthesis (an anabolic process) and β- oxidation of…
Q: The following molecules are lipids. Indicate to which lipid group each belongs.
A: Lipids are the organic substances that are insoluble in water, and soluble in organic solvents like…
Q: how to read this table? like for example, just by looking at the table, it was said that leptin has…
A: Introduction: Separation is brought about through the molecular sieving technique, based on the…
Q: Which of the following statement(s) is/are TRUE about ketogenesis? I. It is favored during a low…
A: Ketogenesis means the synthesis of keto-acids in the human body. Ketoacids are synthesized in the…
Q: 2. Peter does not tolerate lactose in milk. He bought a solution of ß-galactosidase where [Eo] 10.0…
A: First lets find the Vmax value of the enzyme β-galactosidase. Vmax = Kcat×Etotal =525 s-1…
Q: TAG
A: The conversation of biomolecules to its components is called conversation. For one mole of the…
Q: Based on intermolecular attractions, why is honey, which is made up of carbohydrates, 2,000 times…
A: A fluid's viscosity is a measurement of its resistance to deformation at a certain rate. As a…
the answer is 10, but what are the steps?
Step by step
Solved in 2 steps
- Calculate your dilution strategyto make a 625 ng/mLHRP solution from a 12.5 μg/mL stock given the totalvolume of enzyme youwillneed for your condition this week. Note if you are testing pH, you only need to calculate one enzyme dilution because all other pHs will use the same strategy.An enzymatic reaction follows M-M kinetics with Vmax= 2.5 mol m-3s-1and Km = 5 mM.Calculate the time required for 50% conversion of the substrate in a batch reactor if theinitial substrate concentration is0.2 M.Show your calculation steps.Sucrase has an optimum temperature of 37°C and an optimum pH of 6.2. Determine the effect of the following on its rate of reaction: 1) no change 2) increase decrease 3) A. increasing the concentration of sucrase B. changing the pH to 4.0 C. running the reaction at 70°C What are the functions of Allosteric enzymes What are some factors that affects enzyme activity? I. II. III. IV. V. VI. Enzyme activity can be regulated by allosteric enzymes, feedback control, and covalent modifications. T/F Examples of Zymogens are the proteases trypsinogen and chymotrypsinogen. T/F? Trypsin catalyzes the removal of dipeptides from inactive chymotrypsinogen and trypsinogen to give the active proteases chymotrypsin and trypsin. T/F The removal of a polypeptide chain from proinsulin produces the active form of insulin. T/F? A kinase can activate an inactive enzyme by phosphorylation, ie adding a phosphate group. T/F? A phosphatase can activate an inactive enzyme by removal of phosphate. T/F? Identify…
- The hydrolysis of a substrate, S, by an enzyme has been studied in the lab. The following initial rates, vo, were recorded at different concentrations. [S] (M) Vo (M/min) 2.10-10-4 1.20.10-6 4.20-10-4 3.10.10-6 9.30-10-4 6.30-10-6 1.42-10-3 9.10-10-6 A. Determine the rate constants for degradation of the substrate B. What is the rate of reaction at [S] = 1.1.104 M? C. Explain, why enzymes can make reactions go faster? Does enzymes also catalyse the reverse reaction from product to substrate?You need to make a 10 ng/ul solution of enzyme from a 100 mg/ml solution. How will you use a dilution series to do this? You should not pipette less than 1 ul. Any dilutions made need to be 5 ml in volume.8 7- 6- 2- 1- 45 90 135 180 225 270 315 360 405 450 495 540 58! [Substrate] (nM) What is the Vmax of the enzyme data shown above? rate (nM/sec) 3.
- A continuous enzyme reactor consists of a stirred tank of volume 0.01 m3 through which liquid is pumped at 0.02 m3⁄h. The enzyme is immobilised onto non-porous, spherical support particles, and the contents are stirred well to ensure that the composition is uniform and that the particles remain in suspension. A plastic mesh has been placed in front of the exit pipe to prevent the particles from leaving the vessel. The reactor is used to carried out a 98% conversion of a substrate entering at 0.1 kmol m3 ⁄ . Michaelis-Menten kinetics apply, with the following enzyme characteristics: Turnover number = 1 × 10^3 s KM = 5 × 10−3 kmol m3 ⁄ Enzyme RMM = 65,000 (a) If the enzyme is stable, calculate the mass of enzyme that needs to be immobilised. (b) If the enzyme is not stable and experiences exponential denaturation with a half-life of 10 h at the reaction temperature, determine the denaturation constant. (c) How would you expect denaturation to affect the reactor performance?0.0025 0.002 0.0015 0.001 0.0005 10 20 30 40 50 60 70 80 -0.0005 Temperature (C) 1.What trend did you observe for the rate of peroxidase compared to the temperature of the enzyme? 2.What was the optimum temperature tested for the enzyme peroxidase? 3.Did any of the temperatures tested cause the enzyme to denature? Which one? Rate (abs/sec @ 500nm)An enzyme catalysed reaction has a Km of 8 mM and a Vmax of 13 nM.s-1. Use the Michaelis-Menten equation to calculate the reaction velocity when the substrate concentration is 18 mM.
- You measure a Vmax of 123 µM/min when using an enzyme concentration of 11.3 µg/mL in the test tube. You know that the enzyme has a relative molecular mass of 23 kDa. From this information, calculate the turnover number of the enzyme. The turnover number is minute-¹You are working on an enzyme that obeys standard Michaelis-Menten kinetics. You have determined the Vmax to be 0.1 mol/sec and the Km to be 2.5 mM. What would the rate of the reaction be when the substrate concentration is 20 mM? 0.09 MS-1 O 0.133 Ms-1 O 0.18 Ms ¹ 9 Ms-1 O 0.018 Ms-1 0.2 MS-1An enzyme-catalyzed reaction has a Km of 4 mM and a Vmax of 5x10-3 uM/s. What is the reaction velocity when the substrate concentration is: 5 mM 4 mM