An aqueous 2-phase extraction method is used to purify a recombinant fusion peptide made in E. coli. To achieve the most efficient separation, 450 kg of a mixture of 19.7% w/w polyethylene glycol (PEG) and 17.7% w/w potassium phosphate salt in water is used. You notice there are some leftover solutions. You have: - 100 kg of a mixture of 20% w/w PEG in water - 150 kg of a mixture of 20% w/w PEG and 25% w/w salt in water Your supplies are the following: - 200 kg of an aqueous mix of 50% w/w PEG (stock solution) - 200 kg of an aqueous mix of 40% w/w salt (stock solution) - Unlimited water You’re an efficient engineer, so you’re going to use the leftover solutions first (waste not, want not, and all that). To get to that “most efficient separation,” how much of each stock solution and water is needed? You are going to use all of both leftover mixtures first.
An aqueous 2-phase extraction method is used to purify a recombinant fusion peptide made in E. coli. To achieve the most efficient separation, 450 kg of a mixture of 19.7% w/w polyethylene glycol (PEG) and 17.7% w/w potassium phosphate salt in water is used. You notice there are some leftover solutions. You have: - 100 kg of a mixture of 20% w/w PEG in water - 150 kg of a mixture of 20% w/w PEG and 25% w/w salt in water Your supplies are the following: - 200 kg of an aqueous mix of 50% w/w PEG (stock solution) - 200 kg of an aqueous mix of 40% w/w salt (stock solution) - Unlimited water You’re an efficient engineer, so you’re going to use the leftover solutions first (waste not, want not, and all that). To get to that “most efficient separation,” how much of each stock solution and water is needed? You are going to use all of both leftover mixtures first.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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An aqueous 2-phase extraction method is used to purify a recombinant fusion peptide made
in E. coli. To achieve the most efficient separation, 450 kg of a mixture of 19.7% w/w
polyethylene glycol (PEG) and 17.7% w/w potassium phosphate salt in water is used.
You notice there are some leftover solutions. You have:
- 100 kg of a mixture of 20% w/w PEG in water
- 150 kg of a mixture of 20% w/w PEG and 25% w/w salt in water
Your supplies are the following:
- 200 kg of an aqueous mix of 50% w/w PEG (stock solution)
- 200 kg of an aqueous mix of 40% w/w salt (stock solution)
- Unlimited water
You’re an efficient engineer, so you’re going to use the leftover solutions first (waste not,
want not, and all that). To get to that “most efficient separation,” how much of each stock
solution and water is needed? You are going to use all of both leftover mixtures first.
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