Abs undiluted 0.208 Diluted (1/10) 0.105 Diluted (1/20) 0.09 Use the chart above to back calculate the protein concentration of the LDH sample based on the Abs562 measurements of the undiluted and 2 diluted LDH samples. Show calculation for each.
| Abs | |
| undiluted | 0.208 |
| Diluted (1/10) | 0.105 |
| Diluted (1/20) | 0.09 |
Use the chart above to back calculate the protein concentration of the LDH sample based on the Abs562 measurements of the undiluted and 2 diluted LDH samples. Show calculation for each.
Absorption spectroscopy is a spectroscopic method that measure the absorption of radiation by the sample. When radiation of range of wavelength passed through any sample solution and at particular wavelength shows maximum absorption.
The concentration of solution can be calculated using the Beer's Lambert's Law. It states that the Absorbance of a sample solution is directly proportional to the concentration of that sample. So, it enables us to calculate the concentration of a solution using its absorbance.
A = 
C l
Where, A= absorbance
= Molar absorptivity coefficient/constant
C = concentration
l = Pathlength
LDH enzyme catalyzes the conversion of lactate to pyruvate with conversion of NAD⁺ to NADH and also pyruvate to lactate, by conversion of NADH to NAD+. LDH works by the transfer of a hydride from one molecule to another.
Lactate + NAD+ → Pyruvate + NADH
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