ABCD Crime Scene DNA Fingerprint 11. What can you conclude about sample A's involvement in the crime based on the DNA fingerprint? 12. What can you conclude about samples B and D based on the DNA fingerprint? 13. What can you conclude about sample C's involvement in the crime based on the DNA fingerprint? ABCD Paternity DNA Fingerprint 14. What percent of a child's DNA fragments will match their father's? 15. Which of the four men (samples A-D) is the child's father based on the paternity gel? I || | Crime Scene Victim I U| Mother
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- - Explain how DNA fingerprints are created and analyzed using the documents below. Describe the type of data that can be gathered from a DNA fingerprint as it relates to COVID-19. - Identify two individuals from Document A who are likely most closely related. Justify your claim with evidence from the documents. - Examine Document D. Lanes 1 and 9 include DNA fragments of known lengths and are used as reference only. Predict the type of analysis that will be conducted on this DNA profile (suspect identification, relatedness, genetic disease). Justify your prediction with evidence. (Documents A through D are related to this question)DNA Sample : Blood Stain collected from handle of knife blood Bob Sue John Lisa stain 1. What do you notice about the black lines? 2. How do we read the gel electrophoresis? 3. Which sample are we comparing Bob, Sue, John, and Lisa's DNA to? 4. How do they compare?Can you please check my answer and make sure it is correct. Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful? Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…
- Since DNA anaylsis is specific to human body fluids such as blood, why do Crime Scene Investigators even bother with doing field test for biological fluids? Why not submit all samples that might have any kind biological fluid to the lab?Paternity Test: Alanna claims John is the father of Timmy, but Leo thinks that he is the father. All of their DNA samples were taken and cut with the same restriction enzyme and run through a gel. 4. Who is the father of Timmy? 5. How many of Timmy's fragments are the same size as his mother's fragments? 6. Whatactually causes the DNA pieces to move through the gel? 7. Describe the difference you see in the gel between John's DNA and Leo's DNA. Alanna's DNA Timmy's DNA John's DNA Leo's DNAWhat is the size of the DNA sample based on the gel profile below? Make sure to indicate the units. Sample bp 6000 5000 2000 1000 500 250 100 50 20 M 2.35u 4.26u 5.47u 6.67u 7.88u 9.47u 10.68u 12.27u 2.67u 10.26u Dye front: 16u
- Write an analysis of the results, interpreting what you see in the smaller gell focusing on 6A, 6B, 6C, 6D and 6CS ( Crime Scene) DNA fragments in the agrose gell. Who is the guilty suspect and why ?You are a forensic scientist in charge of DNA samples in a case where a man was murdered. You conduct gel electrophoresis on the samples to determine the DNA profile of the samples. The rows of bands have been marked to make it easier to reference the bands. The sample marked 'evidence' came from under the victim's fingernails, likely indicating a struggle. There are two suspects in the case, both of whom are brothers, they are marked suspect 1 and suspect 2, for brother 1 and brother 2, respectfully. Does the evidence collected match the DNA profiles of either brother? If yes, which one? If no, then would it be likely that the actual criminal is a family member of the brothers? If yes, what could the relation be (mom, dad, cousin, aunt, or uncle)? Note: there is a sister in their family, their parents are both still alive, and they have a robust extended family. The brothers are not twins.Questions: Answer questions 1 to 3 about this gel electrophoresis. 1. Which color represents the smallest DNA fragment? (explain) 2. If “A" is the crime scene and B to E are suspects. Do you have any match? (Why or Why not) 3. Is there a possibility that any of the suspect related to the person who committed the crime? Explain.
- Many times a forensic scientist has only a single hair or a single drop of blood for analysis. The amount of DNA in this kind of material is very tiny, and is not enough to use for comparison. In other words, running a single experiment to compare the DNA in a drop of blood with several suspects would use up all of the DNA in that drop of blood. This could be a serious problem if none of the suspects provided a match. How would a forensic scientist increase the amount of DNA available to them, starting with the DNA that would be found in their crime scene sample, such as a drop of blood from a suspect? PCR RFLPS Gel electrophoresis STRSTHe DNA profiles of five people potentially involved in a robbery are shown in DNA Profile #2. The profile on the left side was obtained from DNA belongíng the robber. The four other profiles were obtained from the suspects. Which of the potential suspects is most likely the robber?The results of gel electrophoresis of 4 different DNA samples given in the figure. 16 ul was loaded into each well (6 μL diluted DNA* + 8 μL water + 2 μL sample buffer). The ladder is in lane 5, with the size and amount (ng) in each band indicated. *1:5 dilutions of the original DNA sample were made, and the diluted samples were used for AGE. Q. These are the conclusions made about sizes and concentrations of the original (undiluted) DNA samples from lanes 1 and 2. Are these conclusions reasonable? Explain specifically, and if any of the conclusions are not reasonable, explain why not and what should we conclude instead. Sample in lane 1:size is 2.32 kb concentration of original DNA sample is 9.2 ng/ul Sample in lane 2:size is 4.36 kb concentration of original DNA samples is 100 ng/ul Q. The sample in lane 3 was expected to be about 2 kb in size. What is a possible explanation for the results observed?