a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated. After incubation, 150 colonies appeared. what is the CFU/mL in the original culutre?
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a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated. After incubation, 150 colonies appeared. what is the CFU/mL in the original culutre?
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- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?A bacterial suspension was diluted and 0.1 mL of each of the dilutions was plated in duplicate. The following results were obtained. What was the original concentration? Dilution CFU Counted CFU Counted 10-2 29 24 10-3 1 3 10-4 0 0
- A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.
- Five grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by four successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample?In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?
- 1 ml of urine is added to 2 ml of water. 1 ml is plated. 81 colonies form. What is the CFU/ml of the urine culture?Five grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by four successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample? Assume: 1 g = 1 mLYou are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer of a 10-1 dilution and it has 38 colonies on it. Calculate the number of CFu/mL for this plate.