A) Make 500 ml of a 50 X solution of HEPES buffer. B) Use this (50X HEPES Buffer) to make 1 liter of 0.5X HEPES Buffer solution. Write out how you would do this and include the concentration of each ingredient in the 0.5X Buffer. GIVEN: RECIPE FOR 1X HEPES Buffer (1 Liter) NaCl: 115 mM (FW: 58.44). CaCl2:1.2 mM (FW: 110.98) MgCl2 :1.2 mM (FW: 95.21) K₂HPO4:2.4 mM (FW: 174.18) HEPES: 20 mM (FW: 238.30) Adjust pH to 7.4.

A) Make 500 ml of a 50 X solution of HEPES buffer. B) Use this (50X HEPES Buffer) to make 1 liter of 0.5X HEPES Buffer solution. Write out how you would do this and include the concentration of each ingredient in the 0.5X Buffer. GIVEN: RECIPE FOR 1X HEPES Buffer (1 Liter) NaCl: 115 mM (FW: 58.44). CaCl2:1.2 mM (FW: 110.98) MgCl2 :1.2 mM (FW: 95.21) K₂HPO4:2.4 mM (FW: 174.18) HEPES: 20 mM (FW: 238.30) Adjust pH to 7.4.

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter14: Equilibria In Acid-base Solutions

Section: Chapter Questions

Problem 26QAP: You want to make a buffer with a pH of 10.00 from NH4+/NH3. (a) What must the [ NH4+ ]/[ NH3 ]ratio...

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