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- Describe the concept behind sterilization by fi ltration, including examples of applications.With aid of a component layout diagram, describe the principle of operation of little sister sterilizer.MacConkey agar plates represent ________ medium. a differential a minimal a selective both a differential and a selective both a minimal and a selective
- Can culture media be sterilized in the hot air sterilizer? Why or why not?1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…Define the meaning of Surface sterilization of explants Surface sterilization of explants
- Can culture media be sterilized in the hot air sterilizer? Why or why not? (Answer in not more than 5 sentences)You have a broth medium that you would like to turn into a solid medium in order to do streak plates. What could you add to your media to allow it to solidify? Paragraph В I UAgar is a solidifying agent used in media preparation. What makes it ideal for cultivation of microbes?
- Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.Define sterilization.Give the components, properties and special considerations in preparation (if there's any) on the following media 1. MacConkey Agar 2. EMB Agar 3. HEA 4. SSA