A fellow classmate is planning to study endocytosis at neuronal synapses for their senior thesis. He is interested in what type of molecules neurons endocytose. They propose to use a cell line mutated in the microtubule motor kinesin to keep endocytic vesicles clustered at the synapse where they can be isolated into synaptosomes through differential centrifugation. Your classmate shows you protocols for growing neurons in culture and one that demonstrates the feasibility of isolating synaptosomes. But you know there is something else wrong with this proposal that will keep it from succeeding. What is it?

A fellow classmate is planning to study endocytosis at neuronal synapses for their senior thesis. He is interested in what type of molecules neurons endocytose. They propose to use a cell line mutated in the microtubule motor kinesin to keep endocytic vesicles clustered at the synapse where they can be isolated into synaptosomes through differential centrifugation. Your classmate shows you protocols for growing neurons in culture and one that demonstrates the feasibility of isolating synaptosomes. But you know there is something else wrong with this proposal that will keep it from succeeding. What is it?

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter3: The Plasma Membrane And Membrane Potential

Section: Chapter Questions

Problem 7RE

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Membrane proteins have six possible functions (transport, cell-cell adhesion, sensing signals, enzymatic activity, cell-cell recognition, and attachment to the cytoskeleton). Based on what you know about the entire synapse (which includes the presynaptic membrane, the synaptic cleft, and the postsynaptic membrane), which of those six functions appear to be happening at the synapse? For each specific function provide a specific example of that function and provide a specific example of that function at the synapse.
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