a) b) c) Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. ii) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. Give an advantage of MEKC over CZE.
a) b) c) Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. ii) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. Give an advantage of MEKC over CZE.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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Transcribed Image Text:a)
b)
c)
Describe how an efficient separation of anions can be achieved using capillary zone
electrophoresis (CZE).
Two neutral compounds, A and B are succesfull separated by micellar electrokinetic
chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium
dodecyl sulphate (SDS).
i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the
hydrophobicity property of the compound based on the migration times obtained.
ii)
If SDS is added in a very low concentration (< 50 mM) into the system, explain
the obtained separation.
Give an advantage of MEKC over CZE.
Expert Solution
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Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step 1: Introduce the Techniques of CZE and MEKC
VIEWStep 2: Understand the Principle of Anion Separation in CZE
VIEWStep 3: Analyze Hydrophobicity Based on Migration Times in MEKC
VIEWStep 4: Analyse the Effect of Reduced SDS Concentration in MEKC
VIEWStep 5: Highlight the Advantage of MEKC over CZE
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