_P 24/7 help Suppose you were provided with a culture of 10,000 cells of coli, to which radioactively labelled deoxy-cytosine triphosphate (dCTP) had been added and the cells grown for 20 minutes. The cell culture suspension was then centrifuged, and the cell pellet was collected and analysed. What would happen if each of the original 10,000 cells There will be 20,000 cells, but had replicated once in this culture, in the 20 minutes' time? [Select] There will be 20,000 cells, and all will be radioactive There will be 20,000 cells, but they will have no radioactivity, as the labelled dCTP remains in the supernatent due to being light There will be 20,000 cells, but only the original 10,000 will be radioactive There will be 20,000 cells, but only the new 10,000 will be radioactive
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
please give anser asap
![LP
- 24/7
ly help
Suppose you were provided with a culture of 10,000 cells of coli, to which radioactively labelled deoxy-cytosine
triphosphate (dCTP) had been added and the cells grown for 20 minutes. The cell culture suspension was then
centrifuged, and the cell pellet was collected and analysed. What would happen if each of the original 10,000 cells
had replicated once in this culture, in the 20 minutes' time? There will be 20,000 cells, but K
[ Select]
There will be 20,000 cells, and all will be radioactive
There will be 20,000 cells, but they will have no radioactivity, as the labelled dCTP remains in the supernatent due to being light
There will be 20,000 cells, but only the original 10,000 will be radioactive
There will be 20,000 cells, but only the new 10,000 will be radioactive
Previous
Next ▸](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F119d1bed-9b12-43b4-801e-dd3337ce3687%2Fba216072-83c4-4a9b-9ca4-98aca9258306%2Fm77r1i_processed.png&w=3840&q=75)
Step by step
Solved in 2 steps
