6. Why did the enzyme activity differ in the ice bath compared to the boiling water bath?

Body Structures & Functions
12th Edition
ISBN:9781285695495
Author:Scott
Publisher:Scott
Chapter19: Nutrition
Section: Chapter Questions
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See attached, you have a table that is perhaps required for some of the questions. 
Answer question number 6 in your own words. Do not copy and paste from google

Part 3
5. What was the optimal temperature for amylase? Explain how your iodine test
results support your answer.
5
6. Why did the enzyme activity differ in the ice bath compared to the boiling water
bath?
Part 4
7. What was the optimal pH for amylase? Explain how your iodine test results
support your answer.
8. Pepsin is an enzyme that hydrolyzes proteins in the stomach.
a. The pH in the stomach is 2. What do you think the optimum pH of
pepsin would be?
b. What happens to the activity of pepsin when it enters the small intestine
where the pH is 8?
Transcribed Image Text:Part 3 5. What was the optimal temperature for amylase? Explain how your iodine test results support your answer. 5 6. Why did the enzyme activity differ in the ice bath compared to the boiling water bath? Part 4 7. What was the optimal pH for amylase? Explain how your iodine test results support your answer. 8. Pepsin is an enzyme that hydrolyzes proteins in the stomach. a. The pH in the stomach is 2. What do you think the optimum pH of pepsin would be? b. What happens to the activity of pepsin when it enters the small intestine where the pH is 8?
Lab 5: Enzymes
Results:
Table 1, Effect of enzyme concentration
Amount of lodine Test Results (color)
Amylase
Enzyme
0 drops
3 drops
6 drops
9 drops
Boiling water
bath
37° water
bath
Ice
bath
0 min
water
dark blue
Table 2. Effect of temperature
Temperature
dark blue
dark blue
dark blue
lodine Test
Results
(color)
10 min
dark blue
yellow
Brown
5 min
dark blue
dark brown
brown
light brown
5
6
7
10 min
9
dark blue
Table 3. Effect of pH
pH
light brown
light green
green
5 min
yellow
yellow
yellow
Benedict's
Test Results
(color)
lodine Test
Results (color)
yellow
10 min
bright blue
light blue
yellow
yellow/red
lodine Test
Results (color)
15 min
yellow
yellow
yellow
faint yellow
Transcribed Image Text:Lab 5: Enzymes Results: Table 1, Effect of enzyme concentration Amount of lodine Test Results (color) Amylase Enzyme 0 drops 3 drops 6 drops 9 drops Boiling water bath 37° water bath Ice bath 0 min water dark blue Table 2. Effect of temperature Temperature dark blue dark blue dark blue lodine Test Results (color) 10 min dark blue yellow Brown 5 min dark blue dark brown brown light brown 5 6 7 10 min 9 dark blue Table 3. Effect of pH pH light brown light green green 5 min yellow yellow yellow Benedict's Test Results (color) lodine Test Results (color) yellow 10 min bright blue light blue yellow yellow/red lodine Test Results (color) 15 min yellow yellow yellow faint yellow
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