3. We made 450 mL of 2.5% Luria Broth (LB) for our bacterial cultures. a) What is the final concentration? b) What is the final volume? c) How many grams of LB powder was used to make the LB? Write the equation used to solved this.
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- 3.1) What are the reasons for finding Gram-positive organisms on a MacConkey agar plate? 3.2) What does it mean if the Gram-negatives on the MacConkey agar form red or colourless colonies? 4) A solution contains the following: 5% NaCl, 12 g/l of agar-agar, 0.1 M NaOH (M, = 40 g/mol). Calculate how much of each is needed for 550 ml. Show all 653 PM 18°C NO Back F4 7 8 6 4 5 P R G LOA disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.a) What dilution is created when you dilute 1mL of bacteria into 99mL of water? 10- What is the dilution factor? 99 b) What dilution would you create if you added 1mL of a 10-2 suspension to a 99mL What is the dilution factor? water blank?
- 50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are: A) Acidimetry B) Alkalimetry C) Complexometry D) Iodometry E) Refractometry ● Calcium chloride is available in dosage forms: A) Powder for preparation of injections B) Solution for injection 10% in ampoules C) Tablets of 0.3 g D) Solution for injection 25% in ampoules E) Dry dosed powder
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.Which of the alternatives below indicates techniques used both to purify and to concentrate proteins, without losing the three-dimensional structure? a) diafiltration, affinity chromatography, centrifugation b) diafiltration, salting out, affinity chromatography c) precipitation with organic solvent, diafiltration, centrifugation d) centrifugation, precipitation with organic solvent, salting out4. Which of the following tests for reducing sugar? a) Iodine b) Benedicts c) Biuret d) Emulsion
- 6) The autoclave desired Temperature to kill bacterial spores must be left for at least ____ in order to effectively kill the bacterial spores: a) 5 mins b) 10 mins c) 15 mins d) ½ hr e) 1 hr.7) The autoclave pressure must reach at least ________ in order to effectively kill the bacterial spores: a)15 PSI b) 15 Joules c) 15 KSI d) 15 Lbs e) 15 Atmospheres8) The antibiotic that inhibits the cell wall formation through the -Lactam ring is/are:a) Vancomycin b) Tetracycline c) Penicillin d) Cephalosporin e) c & d9) The sum of all chemical reactions in your body defines: a) anabolism b) metabolism c) catabolism d) Cellular Respiration e) Biosynthesis10) If in a bacterial growth medium waste product accumulates than the bacterial growth stage will be called: a) Death b) Convalescence c) Lag phase d) Log e) Stationary5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts 6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above 7. The temperature to incubate the bacteria in the above mentioned experiment should be __: a) 22 oC b) 37 oC c) 98 oC d) 37 oWith the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE