250 150 2 -250 150 100 -100 75 -75 50 -50 37- 37 25 20 15 10 Std Calf Fibrinogen BSA Std Biorad Biorad 161-0374 serum Transferrin 161-0318 -25 -20 -15 -10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data-measured distance moved and calculated Rr values Item Band Distance moved Rr Log 10 Mr (from Calculated Mr (antilog (mm) graph) of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter16: Gene Expression
Section: Chapter Questions
Problem 38CTQ: A scientist discovers a virus encoding a Protein X that degrades a subunit of the elF4F complex....
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Question
250-
1
2
3
4
5
6
-250
150-
-150
100-
-100
75-
-75
50-
37-
25-
20-
15-
10
10-
Std
Biorad
BSA
Calf
serum
Fibrinogen
Std Biorad
Transferrin
161-0318
161-0374
-50
-37
-25
-20
15
10
2. Measure the distance (in mm) moved for each protein standard band and record
in Table3.6 below.
Table 3.6 Example of table for recording of data - measured distance moved
and calculated Rr values
Item
Band
Distance
moved
(mm)
Rf
Log 10 Mr
(from
graph)
Calculated
Mr (antilog
of the
previous
column)
Sample C
Band 1
Sample F
Band 1
Band 2
Sample B
Band 1
Band 2
Sample T
Band 1
Transcribed Image Text:250- 1 2 3 4 5 6 -250 150- -150 100- -100 75- -75 50- 37- 25- 20- 15- 10 10- Std Biorad BSA Calf serum Fibrinogen Std Biorad Transferrin 161-0318 161-0374 -50 -37 -25 -20 15 10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data - measured distance moved and calculated Rr values Item Band Distance moved (mm) Rf Log 10 Mr (from graph) Calculated Mr (antilog of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1
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