2. Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A.) LPO HPO ΟΙΟ
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- Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity 2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate: i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions. 3. What are the methods of staining?Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.
- II.VIRTUAL MICOSCOPE 4X 4X SPECIMEN 10X 10X 40X 40X 100X 100x COARS FOCUA TME FOOR LCHT AVAN 100X MAGNIFICATIO | 4X N USED IN THE VIRTUAL MICROSCOPE OCULAR MAGNIFICATIO (10X) TOTAL MAGNIFICATIOWhich of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.
- The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.
- 1. Identify features of Dark-field Microscopy (1-2 paragraph) 2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. BaseCalculate below the total magnification for each ocular/objective combination on your microscope. Ocular Objective Total Magnification 1. What is the magnification of the oculars on your microscope? 3. What are the magnifications of the objectives on your microscope?