2. Below is data from an HFR time of entry gene mapping experiment. Create a map of the chromosome with all genes labeled and the location and orientations of the oriT sites. Hfr Strain Data orit gal ton azi lac leu oriT azi ton gal trp cys oriT met ade1 arg cys trp 2 4 ortT met ade2 thr leu lac
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- Information obtained by microscopic analysis of stained chromosomes is used in: radiation hybrid mapping sequence mapping RFLP mapping cytogenetic mappinghelp. 1. To produce a specialized-transducing mycobacteriophage construct, a cosmid bearing the allelic exchange substrate is electroporated in Mycobacterium smegmatis, followed by an incubation at 30°C. True/ False 2. A recombineering system involves the over-expression of recombinase proteins, which allows the use of a short DNA fragment as allelic exchange substrate, but can also be combined with the use of a mycobacteriophage construct. True/ False3 2. The table below was obtained by P1 phage transduction followed by media selection of an E. coli strain lacking the three genes. Determine the map order of the genes given the information provided. Demonstrate CLEARLY which are closer or farther from each other (no numbers needed). 1 2 MAP: Experiment Selected marker Ala+ Gly+ Ser+ Unselected markers (select- ed for by subsequent plating) 27% Ser+, 2% Gly+ 3% Ser+, 1% Ala+ 35% Ala+, 4% Gly+
- Why is 26 incorrect1. Inversion type that produces inv9 and inv12 2.Condition caused by translocation between chromosomes 8 and 2 and what specific type of translocation? 3.Point mutation with no abnormal effect 4.Physical agent that displaces electron from neutral atoms. 5.Formed when nonionizing agent disrupts cytosine-guanine pairing.13-14. A set of Hfr strains transfer markers in the following order. Strain Transfer order JIAF G 1 ED BGFA IJH CE D CED B 13. Which of the following gene orders is correct? A: AFGEDBCHIJ B: ECHIJAFGBD C: E D B G FACH JI D: E D B G FACHIJ E: A F G BD ECHJI 14. Which Hfr strains have F factors inserted in the same orientation? A: 1 and 3 oriented the same way, 2 and 4 both oriented the other way B: 1 and 2 oriented the same way, 3 and 4 both oriented the other way C: 1 and 4 oriented the same way, 2 and 3 both oriented the other way D: 1 oriented one way, 2, 3 and 4 all oriented the other way E: all are oriented the same way 2 UYSerlero
- The DNA fragments show a portion of two nonsister chromatids before crossover. Suppose strand invasion occurs between regions A and B. The heteroduplex extends and forms the Holliday intermediate shown below right. Each dotted line represents one potential plane on which endonucleases could cut the intermediate. DEDE Left chromatid A Right chromatid b A B D Nonsister chromatids before crossover Holliday intermediate Match the chromatids that result from the different ways in which the Holliday intermediate can be resolved. Top chromatid B D Bottom chromatidChromosome initiated has following sequence ab*cdefg *= centromere draw the chromosome that would result from the following mutations a paracentric inversion that includes defg b peri centric inversion of bcdeFor fruit flies, N=4 and 2N=8 a. Sketch a dividing fruit fly cell which is in prophase I. b. Sketch a dividing fruit fly cell which is in metaphase of mitosis. A 35 year old man developed increasing pain in his left thigh and a marked limp, over 3 weeks. Five years he had undergone a nephrectomy for a renal carcinoma. An isotope showed metastasis in his right lateral ribs and left femur and he had been taking sunitinib. Assuming you are the attending physician, what is the most appropriate next step in his management?
- . a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+, Xyl+,Tyr+, Arg+, His+, Mal+, and Strs. Describe anappropriate bacterial strain to be used as theother partner in this mating.b. In an Hfr × F− cross, the pyrE gene enters therecipient in 5 minutes, but at this time point thereare no exconjugants that are Met+, Xyl+, Tyr+,Arg+, His+, or Mal+. The mating is now allowed toproceed for 30 minutes and Pyr+ exconjugants areselected. Of the Pyr+ cells, 32% are Met+, 94% areXyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and71% are Mal+. What can you conclude about theorder of the genes?The minichromosome maintenance complex is found in eukaryotes. True FalseSite specific recombination using Cre-Lox system shown below will result in A laxP toaP laxP JoxP Inversion of the transgene Deletion of the transgene Translocation of the transgene Transformation of the transgene