2) Assume that you made an error during your staining procedure. You used safranin as the primary stain and malachite green as counterstain. How would your cells appear at the end of the staining of the staining technique?
Q: Explain the reasons you would use a negative stain?
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A: Direct staining is the process to make slides using a single stain or dye.
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…
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Q: What are the principle and basic concepts of DIFFERENTIAL staining?
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Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
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Q: . What is the purpose of fixation in cytological staining?
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Q: What is the principle of Giemsa staining and components of Giemsa stain?
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A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: What are the principle and basic concepts of NEGATIVE staining?
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Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: I Why does't a negative stain colorize the cells in the smear?
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Q: why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
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Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
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Q: The benefits of the negative stain include: Mark all that apply: O No shrinkage or distortion of…
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Q: What is an example of a negative stain?
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Q: What are the principle and basic concepts of Simple staining
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Q: Which step of the gram stain procedure is most critical?why?
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Q: Why don't you heatfix a negative stain slide?
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Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
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Q: For What purpose is Giemsa stain used for?
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A:
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
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Q: How can the thickness of the smear affect the staining procedure?
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- In four steps, describe the simple staining procedure.Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cellsWhat are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
- The benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the backgroundGive three situations where the negative staining procedure would be used.This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordant
- Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixingThe benefits of the negative stain include: Mark all that apply: O No shrinkage or distortion of cells due to no heat or methanol fixing Because the cells don't take up stain they don't get shrunk or distorted O One can more accurately determine cell size and shape because the cells stand out against the background One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells O Negatively charged dyes are safer to work with than positively charged dyes IMicrosoft B 11:32 AHow will the staining appear if if the destaining step were done at high temperature?