1. Under the conditions listed above, will rrgBA be expressed? [Select] 2. Under the conditions (media) listed above, are your recombinant cells likely to be pathogenic? [Select] 3. Is the media you are using selective? Yes

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter3: Basic Hemostasis
Section3.6: D-dimers
Problem 1.1CS
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blaA
ori
lacl
Plac
rrgB
rrgA
lacZYA
Recombinant Plasmid "B"
Transcribed Image Text:blaA ori lacl Plac rrgB rrgA lacZYA Recombinant Plasmid "B"
You transform Plasmid B into E. coli and plate onto Glucose Minimal Media + X-gal + Amp.
1. Under the conditions listed above, will rrgBA be expressed?
[Select]
2. Under the conditions (media) listed above, are your recombinant cells likely to be
pathogenic? [Select ]
3. Is the media you are using selective? Yes
4. Is the media you are using differential? Yes
5. Is the media you are using complex? No
6. What color colonies would you expect with cells expressing this plasmid?
[Select]
Transcribed Image Text:You transform Plasmid B into E. coli and plate onto Glucose Minimal Media + X-gal + Amp. 1. Under the conditions listed above, will rrgBA be expressed? [Select] 2. Under the conditions (media) listed above, are your recombinant cells likely to be pathogenic? [Select ] 3. Is the media you are using selective? Yes 4. Is the media you are using differential? Yes 5. Is the media you are using complex? No 6. What color colonies would you expect with cells expressing this plasmid? [Select]
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